水貂犬瘟热病毒受体——信号淋巴细胞激活因子的细胞定位及组织分布  

Cellular Localization and Immunohistochemical Demonstration of A Sort of Canine Distemper Virus Receptor Named Signalling Lymphocyte Activation Molecule in Minks

作　　者：黄娟[1] 王聪[1] 张洪亮[1] 单虎[1] 

机构地区：[1]青岛农业大学山东省高校预防兽医学重点实验室,青岛266109

出　　处：《畜牧兽医学报》2014年第2期289-294,共6页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：山东省自然科学基金(ZR2010CL023);国家科技基础性工作专项(2012FY111000)

摘　　要：为探讨犬瘟热病毒受体——信号淋巴细胞激活因子(SLAM)的细胞定位、在水貂组织中的分布及病毒感染对受体表达水平的影响,本研究采用间接免疫荧光法(IFA)进行了SLAM细胞定位。以IFA检测感染水貂肝、脾、肺、肾、脑组织的犬瘟热病毒抗原,以半定量PCR和间接免疫组化法(IHA)检测健康水貂及感染水貂SLAM在以上组织中的分布。结果表明,在Vero-SLAM细胞膜及细胞质内可见SLAM特异性荧光;感染水貂肝、脾、肺、肾、脑组织均可见大量犬瘟热病毒抗原阳性细胞,健康水貂及感染水貂肝、脾、肺、肾、脑组织SLAM PCR阳性,IHA检出数量不一的SLAM阳性着色细胞,且感染水貂相应组织的SLAM阳性着色细胞显著多于健康水貂(P<0.05);感染水貂的肾、脾、肺组织中犬瘟热病毒阳性细胞显著多于SLAM阳性着色细胞。说明SLAM受体表达于细胞膜和细胞质,在肝、脾、肺、肾、脑组织中均有分布;犬瘟热病毒感染引起SLAM受体表达的上调;在水貂体内除了SLAM受体,还有其它犬瘟热病毒受体存在。In order to study cellular localization,tissue profile and regulation of signalling lymphocyte activation molecule(SLAM),a receptor of canine distemper virus(CDV),indirect immunofluorescence assay(IFA)was conducted to detect SLAM expression in Vero cells,IFA was also used to detect CDV-antigen in liver,lung,spleen,kidney and brain of CDV-infected minks;semiquantitive PCR and Indirect Immunohistochemical assay(IHA)was selected to detect SLAM expression in referred tissues of minks with and without Distemper.The results showed that viridis fluorescence was appeared in cell membrane and cytoplasm of Vero-SLAM cells,CDV and SLAM positive reaction was appeared in cell membrane and cytoplasm of liver,lung,spleen,kidney and brain of CDV-infected minks;In minks with early distemper lesions characterized by presence of the virus,higher numbers of SLAM-expressing cells were found in multiple tissues recognized as targets of CDV compared with those in control minks,more CDV-positive cells were observed than SLAM-expressing cells.These findings suggest that SLAM is expressed in cell membrane and cytoplasm of minks,especially in lung.Virus infection is associated with up-regulation ofSLAM,and there is other receptor for CDV in minks.

关 键 词：犬瘟热病毒 受体 信号淋巴细胞激活因子(SLAM) 细胞定位 组织分布 

分 类 号：S858.92[农业科学—临床兽医学]