鸡与鹌鹑杂交种早期胚胎与性分化相关的ER等基因的表达  

Expression Analysis of Genes Related to Sexual Differentiation during the Early Development of Chicken-Quail Hybrid Embryos

作　　者：刘贤侠[1] 冯欣璐[1] 赵宗胜[1] 李大全[1] 高树[1] 

机构地区：[1]石河子大学动物科技学院,石河子832003

出　　处：《畜牧兽医学报》2014年第2期320-326,共7页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家自然科学基金项目(30660125)

摘　　要：本研究旨在对影响鸡与鹌鹑杂交早期胚胎发育及性分化4个基因的表达进行检测,初步判定其对杂交种胚胎性别分化和早期死亡可能起到的作用。研究选取杂交早期胚胎发育不同时间点(72、78、84、90、96、102、108、114、120、144、168和192h)活胚全胚,在鉴定杂交种早期胚胎性别的基础上,采用Real-time PCR方法检测雌雄杂交早期胚胎发育过程中不同时间点性分化相关基因的表达。结果表明,鸡与鹌鹑杂交胚胎中3β-HSD和ER基因雌性的表达量总体显著高于雄性。3β-HSD表达量雄性胚胎在72h最低,雌性和雄性杂交胚胎96h表达量最高。在90h内雌、雄性杂交胚胎P-450c17基因表达水平较高,在78h达到最高峰,在90h内的各个时间点雌性与雄性比较差异极显著(P<0.01)。杂交胚胎P-450arom基因在雌性可以检测到表达,但是雄性不能检测到表达。在杂交胚胎发育早期ER的表达有2个时间点是高峰,分别在72和108h。This study aimed to detect expression of genes related to early embryonic development and sexual differentiation in the chicken-quail hybrids.First,the whole embryos were collected during the hybrid early embryonic development at different times(72,78,84,90,96,102,108,114, 120,144,168and 192h).Then,based on the identification of early hybrid embryo sexes,Real-time PCR method was used to detect the expressions of sex differentiation associated genes(3β-HSD, P-450c17,P-450aromand ER)during the embryonic development of male and female hybrids at different times.The results showed that in chicken-quail hybrid embryos,both of 3β-HSDand ER genes expression in females were generally significantly higher than that in males.The lowest expression of 3β-HSD occured at 72hin male,while the highest expression occurred at 96hin female and male embryos.Within 90h,the gene expression level of the male and female hybrid embryos P-450c17was higher,peaking at 78h,and there were significantly differences(P<0.01)in females and males at each time point within 90h.P-450aromhybrid embryo gene expression could be detected in females,but the expression of the males could not be detected.There were two peaks,respectively,at 72and 108hexpression of ER gene in early hybrid embryonic develop-ment.

关 键 词：鸡与鹌鹑杂交种早期胚胎 ER基因 表达分析 

分 类 号：S831[农业科学—畜牧学] S837[农业科学—畜牧兽医]