APC在羊驼皮肤组织中的表达和定位分析  

Expression and Location Analysis of APCin Alpaca Skin

作　　者：张丹丽[1] 田雪[2] 范瑞文[1] 于秀菊[1] 宋云飞 董常生[1] 

机构地区：[1]山西农业大学动物科技学院,太谷030801 [2]河南师范大学水产学院,新乡453007 [3]华大基因,兰州730000

出　　处：《畜牧兽医学报》2014年第2期327-332,共6页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家"863"计划(2013AA102506);公益性行业(农业)科研专项(201303119);农业部"948"项目(2011-Z33)

摘　　要：本试验旨在研究大肠腺瘤样息肉(APC)在不同毛色羊驼皮肤中的表达及定位情况,探究其与毛色之间的关系。本试验采用实时荧光定量PCR方法检测APC在棕色和白色羊驼皮肤中的mRNA表达情况;采用免疫组织化学方法研究其蛋白的定位及表达水平。实时荧光定量PCR结果显示,APC在棕色羊驼皮肤组织中的相对基因表达量是白色羊驼皮肤组织的5.042 2倍;免疫组织化学结果显示,APC在羊驼皮肤毛囊的毛球及毛根处和表皮部位呈阳性表达;且在棕色羊驼中的表达量高于白色羊驼,提示APC可能参与羊驼被毛颜色的形成。To study the function of APCgene in alpaca coat color formation,the expression and localization of APCin different alpaca coat color were investigated.Quantitative real-time PCR(QRT-PCR)and immunohistochemistry were used to detected the mRNA expression level and the location of APC protein in brown and white alpaca skin.The gene expression quantity of APC in brown alpaca skin was 5.042 2times than white one.The results of immunohischemistry revealed that the APC positive signals were found in hair bulb,hair root and epidermis in alpacas. Moreover,the average protein expression of APC in brown alpaca skin was higher than that in white one based on the average optical density(P<0.05).Based on the results,it indicated that APC may involve in alpaca coat color formation.

关 键 词：APC 羊驼 毛囊 实时荧光定量PCR 免疫组织化学 

分 类 号：S824[农业科学—畜牧学]