间接ELISA和竞争ELISA检测小反刍兽疫病毒抗体的比较  

Comparison of Indirect ELISA and Competitive ELISA for Detecting Peste des Petits Ruminants Virus Antibody

作　　者：王净[1,2] 李刚[2] 史利军[2] 

机构地区：[1]河北北方学院中国农业科学院,张家口075131 [2]北京畜牧兽医研究所,北京100193

出　　处：《畜牧兽医学报》2014年第2期333-336,共4页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家自然科学基金(31172342);国家科技支撑计划(2013BAD12B05);国际合作项目(D32025/17453);国家公益性行业科研专项(200903037)

摘　　要：旨在比较检测小反刍兽疫抗体的间接ELISA和竞争ELISA方法,通过诱导表达小反刍兽疫病毒(PPRV)N蛋白,并以此作为包被抗原,检测94份绵羊和25份山羊血清样本。结果显示间接ELISA与标准竞争ELISA试剂盒的符合率为74.8%,竞争ELISA与标准竞争ELISA试剂盒的符合率为96.6%。竞争ELISA比间接ELISA方法检测结果可信度高,适于临床推广应用。The indirect ELISA and the competitive ELISA method were compared in the detection of serum antibody against Peste des Petits Ruminants Virus(PPRV).The PPRV N protein to be used as a coating antigen was expressed in BL21and purified by Ni Sepharose FF column.Ninetyfour sheep and twenty-five goats serum samples were detected by the ELISA methods based on the recombinant N protein.The coincidence rate of the indirect ELISA and the competitive ELISA kit was 74.8%,the coincidence rate of the competitive ELISA and the competitive ELISA kit was 96.6%.The detection results showed that the competitive ELISA is more reliable than the indirect ELISA method,and the competitive ELISA is suitable for clinical application.

关 键 词：小反刍兽疫病毒 N蛋白 间接ELISA 竞争ELISA 

分 类 号：S855.3[农业科学—临床兽医学]