7.0～18.5d猪胚胎的形态学研究  

Morphological Study of Porcine Embryos at 7.0-18.5 Days Postcoitum

作　　者：孙瑞珍[1,2] 刘世超[2] 胡魁[2] 金连弘[1] 雷蕾[1] 刘忠华[2] 

机构地区：[1]哈尔滨医科大学组织学与胚胎学教研室胚胎与干细胞工程校重点实验室,哈尔滨150081 [2]东北农业大学生命科学学院,哈尔滨150030

出　　处：《畜牧兽医学报》2014年第1期23-30,共8页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家重点基础研究项目973课题(2011CB944202;2009CB941002)

摘　　要：本研究旨在观察猪胚胎的外观形态学变化以及器官原基的发生,为器官发生研究及建立人类疾病模型提供基础。收集7.0~18.5d猪胚胎,通过体式显微镜观察外观形态学;石蜡切片、HE染色观察14.0~18.5d胚胎器官原基发生。结果表明,7.0~7.5d囊胚孵化;12.0~12.5d形成线性胚胎,进入原条期;14.0~14.5d进入体节期。器官原基跟踪显示,肾的发生:15.0~15.5d形成前肾,16.0~16.5d转变成中肾,18.0~18.5d仍处于中肾阶段;脑的发生:15.0~15.5d前、中脑清晰,18.0~18.5d可见4个脑泡,出现端脑;眼的发生:15.0~15.5d可见视泡,18.0~18.5 d,可见视杯;胃和肝的发生:16.0~16.5 d形成胃芽、肝芽,18.0~18.5d形成肝索;心脏的发生:18.0~18.5d可见心房、心室和瓣膜。18.0~18.5 d观察到支气管树。上述结果表明,猪胚胎发育在15.0~18.5d时心、肝、肾、脑、肺、胃及肢芽等原基已发生。Through investigating morphological changes and organ primordia formation of the porcine embryos,we want to lay the foundation for organgenesis study in pig,and for utilization of pigs as the model of human disease.Porcine embryos from day 7.0 to 18.5 post-insemination were collected to observe appearance of embryos by stereomicroscopy.Organ primordial of 14.0-18.5 d embryos were inquired using paraffin section and HE staining.The results showed that blastocysts had hatched from zona pellicuda at 7.0-7.5 d.The majority of conceptuses were filamentous,and the primitive streak emerged at 12.0-12.5 d.And embryos were in somite stage at 14.0-14.5 d.The data of tracking organ primordial were shown as follows.For renal formation,the pronephros appeared at 15.0-15.5 d,and the mesonephros emerged at 16.0-16.5 d and remained at 18.0-18.5 d.For brain development,the prosencephalon and the mesencephalon were clearly visible at15.0-15.5 d.Four brain vesicles and telencephalon were observed at 18.0-18.5 d.For eyes formation,optic vesicle appeared at 15.0-15.5 d,and optic cup was showed at 18.0-18.5 d.For stomach and liver,the buds emerged at 16.0-16.5 d,and liver bud transformed into hepatic cord at 18.0-18.5 d.For heart generation,atrium and ventricle as well as valves were formed at 18.0-18.5 d.The results showed that bronchial tree was observed at 18.0-18.5 d.These results indicate that heart,liver,kidney,brain,lung,stomach,limb buds,etc.are beginning to form during 15.0-18.5 d.

关 键 词：形态学 器官原基 猪 

分 类 号：S828[农业科学—畜牧学]