毒害艾美尔球虫感染对雏鸡肠组织热休克蛋白-70表达的影响  

Effect of Infection of E.necatrixon Heat Shock Protein(HSP)-70 Expression in Intestinal Tissue of Broiler Chickens

作　　者：翟杰[1] 吕志慧[1,2] 马春霞[1,3] 高雪丽[1] 刘超男[1] 王全杰[1] 吕晓萍[1] 郑世民[1] 

机构地区：[1]东北农业大学动物医学学院,哈尔滨150030 [2]遵义医学院珠海校区,珠海519041 [3]广西兽医研究所,南宁530001

出　　处：《畜牧兽医学报》2014年第6期1004-1010,共7页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：黑龙江省教育厅项目(12531015)

摘　　要：将90只海兰褐雏鸡分为对照组、毒害艾美尔球虫(E.necatrix)1次感染组和2次感染组,应用SP免疫组化法,通过对热休克蛋白(HSP)-70表达的检测,较全面系统地研究了E.necatrix感染雏鸡后,其肠组织HSP-70表达的动态变化。研究发现,雏鸡初次感染E.necatrix后第5~9天,其肠组织HSP-70表达均不同程度的低于对照雏鸡,其中十二指肠、空肠和盲肠显著或极显著低于对照雏鸡(P【0.05或P【0.01),之后迅速上升,于第14天高于对照雏鸡并达峰值;E.necatrix二次攻击性感染雏鸡后,其十二指肠、空肠HSP-70表达呈现'先显著低于对照雏鸡,随后上升',并逐渐高于对照雏鸡。而E.necatrix1次大剂量感染雏鸡后,其肠组织HSP-70表达则于第6、10、15天高于对照雏鸡。表明E.necatrix初期感染雏鸡肠组织HSP-70表达受到一定程度的抑制,而E.necatrix大剂量感染雏鸡后,HSP-70表达的增高与E.necatrix对机体的损害程度密切相关。该项研究为进一步探讨球虫的致病机制提供了重要参考依据。Ninety chickens were divided into E.necatrix one time infection group,E.necatrix twice infection group,and control group.We researched dynamic change of HSP-70expression in intestinal tissue of chickens infected with E.necatrixapplying immunohistochemical method.The results showed that the HSP-70expression in intestinal tissue of twice infection chickens were lower than the control chickens by different degrees at the 5th to 9th days after primer infection,the HSP-70expression of duodenum,jejunum and caecum were significant or very significant lower than control chickens(P<0.05or P<0.01),later rising rapidly,higher than control chickens at the 14th day and reached the peak;the HSP-70expression in duodenum and jejunum of chickens showing a trend of significantly lower than control chickens at first,then gradually rising and higher than control chickens after the second challenge infection.But,the HSP-70expression inintestinal tissue of E.necatrixone time infection chickens was higher than control chickens at the6th,10th,15th day.These indicate that the primer infection have a certain degree of inhibition of HSP-70expression in intestinal tissue of chickens,and the increase of HSP-70expression is closely related with the degree of damage on the body with E.necatrixinfection.This study provides important reference for the further study of pathogenic mechanism of coccidian.

关 键 词：雏鸡 E.necatrix 肠组织 HSP-70 

分 类 号：S858.31[农业科学—临床兽医学]