猪胰高血糖素样肽-2对结肠炎小鼠肠道损伤的保护作用研究  

The Therapeutic Effect of Porcine Glucagon-like Peptide-2 in a Murine Model of Experimental Colitis

作　　者：齐珂珂[1] 吴杰[1] 徐子伟[1] 

机构地区：[1]浙江省农业科学院畜牧兽医研究所,杭州310021

出　　处：《畜牧兽医学报》2014年第6期1011-1017,共7页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：现代农业产业技术体系(CARS-36);国家青年科学基金项目(C170105/31101724)

摘　　要：猪胰高血糖素样肽-2(porcine glucagon-like peptide-2,pGLP-2)能够特异性地促进肠黏膜生长与损伤后修复,本试验研究了葡聚糖硫酸钠(dextran sulfate sodium,DSS)致小鼠结肠炎模型下pGLP-2对肠道损伤的修复效果,为其治疗各种因素引起的仔猪肠道损伤和功能紊乱提供了试验依据。18只BALB/c小鼠随机分为3组,DSS组小鼠饮用3%DSS建立小鼠结肠炎模型,DSS+pGLP-2组饮用3%DSS且连续7d腹膜外注射pGLP-2,饮水组小鼠正常饮水。试验期10d。结果表明,与饮水组相比,DSS组小鼠体重、结肠紧密连接闭锁小带(zonula occludens-1,ZO-1)基因的相对表达量显著降低,注射pGLP-2能够显著抑制体重降低和增加ZO-1的表达量;与饮水组相比,DSS组显著增加了结肠炎性评分、髓过氧化物酶(myeloperoxidase,MPO)活性和白细胞介素-6(interleukin-6,IL-6)、白细胞介素-10(interleukin-10,IL-10)和干扰素-γ(interferon-γ,INF-γ)的相对表达量,注射pGLP-2极显著降低了结肠炎性评分、MPO活性和IL-6、IL-10和IFN-γ的表达量。结果提示,pGLP-2可抑制结肠炎小鼠的炎性病变,为其治疗肠道疾病提供了试验依据。As an intestinal-specific trophic factor,porcine glucagon-like peptide-2(pGLP-2)could stimulate intestinal mucosal growth and reduce the severity of injury specificity.In order to provide a potential therapeutic agent for intestinal dysfunction and damage,the protective effects of pGLP-2for colonic injury were measured in dextran sulfate sodium(DSS)-induced colitis in mice.Eighteen BALB/c mice were randomly assigned to three treatments for 10days,mice of DSS group were fed 3%(w/v)DSS through their drinking water for 10days from first day of experiment,mice of DSS+pGLP-2group were extraperitoneally injected with pGLP-2daily from day 3to day 9and drink 3% DSS.The water group received water normally.Results were as follows:DSS-induced colitis clearly decreased body weight and relative expression of zonula occludens-1in the colon,which was significantly reduced by treatments with 3% DSS-pGLP-2.Administration of pGLP-2to DSS-mice preserved normal colonic mucosal architecture and significantly decreased myeloperoxidase activity,the relative expression of interleukin-6,interleukin-10and interferon-γwhich induced by DSS.These results showed that pGLP-2could reduce the severity of colonic in-jury in murine colitis.The potencies of this product highlight its potential as therapeutic agent for intestinal diseases in piglet.

关 键 词：pGLP-2 DSS 结肠炎 小鼠 

分 类 号：S856.4[农业科学—临床兽医学]