肠道上皮氧化应激细胞模型的研究进展  

The Research Progress in Intestinal Epithelial Oxidative Stress Cell Model

作　　者：蔡旋[1] 王静娴[1] 陈小连[2] 郭奇[1] 朱丽慧[1] 徐建雄[1] 

机构地区：[1]上海交通大学农业与生物学院上海兽医生物技术重点实验室,上海200240 [2]江西省农业科学院畜牧兽医研究所,南昌330200

出　　处：《畜牧兽医学报》2014年第3期337-346,共10页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家青年自然基金项目(31201806)

摘　　要：肠上皮细胞极易受氧化的损伤,研究肠上皮细胞损伤有构建动物模型和细胞模型2种做法,单纯使用动物模型研究有成本高、不稳定、难以进行机理研究等弊病,构建合适的细胞培养模型作为动物模型的补充显得十分必要。目前常见的氧化应激造模方式有物理方法(如辐射、缺氧/复氧)及化学方式(如添加过氧化氢、(次)黄嘌呤/黄嘌呤氧化酶)等,每种造模方式都有其适用的模拟条件,实际研究时需根据研究者的研究内容进行选择。常用的肠上皮细胞有原代培养细胞和传代细胞2类,原代培养细胞能较真实反映肠上皮细胞特点,但有操作繁琐、无法长期使用等缺点,传代培养细胞种类较多,需要研究者针对研究的种属进行选择。Intestinal epithelial cells are easily injured by oxidative stress in many ways.There are two methods to study intestinal epithelial cells injure:using animal model and/or cell model.But there are some disadvantages in using animal model only,such as high cost,data unsteadiness and difficult to study mechanism.So,to develop a suitable cell model for intestinal epithelial cells oxidative stress research is necessary.Now,the most oxidative stress inducing method used include physical method(such as radiation,anoxia/reoxygenation)and chemical process(such as add hydrogen peroxide or(hypo-)xanthine/xanthine oxidase).The every method has its own application condition,so the reseachers should select the most suitable one according to the research objectives.The most intestinal epithelial cells used can be classed into two main types:primary culture cells and subculture cells.The primary culture cells can reflect the characteristics of intestinal epithelial cells in vivo,but the intricacy and onerous operation limit their widely application.There are a lot types of subculture cells.They have different source and characteristics.The reseachers should select carefully.

关 键 词：肠道 上皮细胞 氧化应激 细胞模型 

分 类 号：S852.21[农业科学—基础兽医学]