乌骨鸡J亚群白血病的综合诊断及病毒分子生物学特性研究  

Study on Comprehensive Diagnosis and Molecular Characterization of Subgroup J Avian Leukosis Virus from Silkies

作　　者：秦四海[1] 刘立涛[2] 岳瑞超[2] 李宁[2] 屈亚锦[2] 王广文[2] 胡东方[2] 刘思当[2] 

机构地区：[1]临沂大学生命科学学院,临沂山276000 [2]东农业大学动物科技学院,泰安271000

出　　处：《畜牧兽医学报》2014年第3期458-462,共5页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家自然科学基金(31172296)

摘　　要：乌骨鸡是中国特有的优良品种,近年来聊城地区死淘的产蛋种鸡常见肝、脾肿大的肿瘤性疾病。2012年12月收到聊城某乌骨种鸡场送检的6只病死鸡。通过剖检观察、病理组织学检查、PCR检测及病毒分离鉴定,最终确定为J亚群白血病病毒感染所致的髓样细胞瘤和血管瘤。设计4对引物,对病毒全基因序列进行扩增,得到全长为7 647bp,其中gag长2 016bp,pol基因长2 622bp,env长1 689bp。跨rTM和DR区有205bp的缺失,相当于原型株HPRS-103的7 049—7 253bp位置;在先导区序列leader区域中有1个19bp的插入序列。分离株与英国原型株HPRS-103差异较大,但与近几年国内商品蛋鸡的分离株相似性很高,可能来自商品蛋鸡。The Silkie is a typical Chinese breed of chicken.In recent years,numbers of Silkies eliminated from farms in Liaocheng,were found to be infected with tumor diseases,showing hepatomegaly and splenomegaly.On Dec.2012,6dead Silkies from a parent farm were found hepatomegaly and splenomegaly with tumor-like nodules by necropsy.By histological detection,PCR testing, virus isolation and identification,ultimately the Silkies were diagnosed myelocytoma and hemangioma,infected by avian leukemia virus subgroup J(ALV-J).To obtain virus genome sequences, four pairs of primers were designed.And finally 7 647bp sequence,include gaggene(2 016bp), pol gene(2 622bp)and env gene(1 689bp)were obtained.There was a 205bp deletion cross rTM(redundant TM)and DR(direct repeat)area,equivalent to 7 049-7 253position of the prototype ALV-J strain HPRS-103.In the leader sequence of 5′UTR(untranslated region),there was a 19bp insertion sequence.The isolate was quite different with the British prototype strain HPRS-103,but was highly similar with stains isolated from commercial layers in recent years.Therefore,this isolate may come from commercial layer,which will bring great challenge to the control.

关 键 词：乌骨鸡 J亚群禽白血病 髓样细胞瘤 血管瘤 全基因序列分析 

分 类 号：S852.659.3[农业科学—基础兽医学]