丝胶对2型糖尿病大鼠胰岛细胞胰岛素蛋白表达的作用  

Effects of sericine on insulin protein expression in islet cells of type 2 diabetes mellitus rats

作　　者：李健[1] 刘东慧[2] 宋成军[2] 陈志宏[2] 

机构地区：[1]承德市中心医院耳鼻喉科,河北承德067000 [2]承德医学院人体解剖学教研室

出　　处：《中国老年学杂志》2014年第6期1549-1550,共2页Chinese Journal of Gerontology

基　　金：河北省自然基金项目(H2012406018);河北省教育厅项目(2006301);河北省科技厅项目(08276101D-19)

摘　　要：目的观察丝胶对2型糖尿病(T2DM)大鼠胰岛β细胞胰岛素蛋白表达的影响。方法 36只雄性SD大鼠随机分为正常对照组、糖尿病模型组和丝胶治疗组,每组12只大鼠。链脲佐菌素腹腔注射建立T2DM大鼠模型并给予丝胶(2.4 g/kg)灌胃。SP免疫组织化学染色法观察大鼠胰岛细胞胰岛素蛋白的表达情况。结果胰岛素蛋白免疫阳性产物位于胰岛β细胞胞质,呈棕黄色颗粒状。糖尿病模型组大鼠胰岛β细胞胰岛素蛋白的表达水平为(0.392±0.017),明显低于正常对照组大鼠(0.729±0.017)(P<0.01);丝胶治疗组大鼠胰岛β细胞胰岛素蛋白的表达水平为(0.619±0.039),明显高于糖尿病模型组大鼠(P<0.01)。结论丝胶可通过上调胰岛素蛋白的表达保护糖尿病时胰岛β细胞损伤。Objective To observe the effects of sericine on insulin protein expression in islet cells of type 2 diabetes mellitus rats. Methods 36 male SD rats were randomly divided into 3 groups with 12 rats in each group: normal control,diabetes mellitus model and sericine treatment groups. Type 2 diabetes mellitus rat model was made by continuous intraperitoneal injection of streptozotocin,and then the rats were lavaged with sericine( 2. 4 g / kg). SP immunohistochemical stain was used to observe insulin protein expression in islet cells. Results Insulin protein positive immunoreactive products were brown and yellow granules and located in cytoplasm of islet beta cells. Compared with normal control rats( 0. 729±0. 017),the insulin protein expression in beta cells of rats in diabetes mellitus model group( 0. 392±0. 017) obviously was decreased( P<0. 01). The insulin protein expression in beta cells of rats in sericine treatment group( 0. 619±0. 039) was obviously higher than that of diabetes mellitus model group( P<0. 01). Conclusions Sericine can protect islet beta cells by up regulating insulin protein expression during diabetes mellitus.

关 键 词：丝胶 2型糖尿病 胰岛Β细胞 胰岛素蛋白 

分 类 号：R587.1[医药卫生—内分泌]