软肝升白颗粒对小鼠肝纤维化的影响  

Effects of Ruanganshengbaikeli on hepatic fibrosis of mice

作　　者：刘金霞[1] 郭争荣[2] 孙殿兴[3] 吴松柏[1] 

机构地区：[1]承德医学院,河北承德050098 [2]石家庄市人民医院 [3]石家庄白求恩和平医院肝病科

出　　处：《中国老年学杂志》2014年第5期1275-1277,共3页Chinese Journal of Gerontology

基　　金：国家自然科学基金项目(No.30872255);中国肝炎防治基金会王宝恩肝纤维化研究基金(No.20070001)

摘　　要：目的探讨肝组织肝细胞生长因子(HGF)及其受体c-Met mRNA的表达水平及软肝升白颗粒治疗小鼠纤维化的作用。方法选择100只雄性BABL/C小鼠,80只给予0.03%硫代乙酰胺(TAA)饮用水喂养,20只为正常对照组(E组)。20 w后造模成功,并随机分为A、B、C和D组,每组20只。A组小鼠给予软肝升白颗粒8 g·kg-1·d-1,B组小鼠给予4 g·kg-1·d-1,C组小鼠给予鳖甲软肝片0.8 g·kg-1·d-1,D组和E组分别给予等体积的生理盐水,灌胃1次/d。在治疗后4、8 w分别处死小鼠各10只,检测血清透明质酸(HA)、粘连蛋白(LN),同时应用RT-PCR技术检测肝组织中HGF及其受体c-Met mRNA的表达量。结果在给予软肝升白颗粒治疗后,治疗组小鼠转氨酶、HA和LN与模型对照组和鳖甲软肝片组均存在统计学差异(均P<0.05),治疗组HGF/c-Met mRNA表达显著高于对照组(P<0.05)。结论软肝升白颗粒治疗小鼠肝硬化模型后,能够降低转氨酶和肝纤维化指标,而且存在时间和剂量依赖性,可能与其HGF/C-Met mRNA高表达有关。Objective To explore the effect of Ruanganshengbaikeli on hepatic fibrosis of mice. Methods 180 BABL/c mice were established hepatic fibrosis models induced by TAA,then randomly divided into 4 groups. Group A and B were administrated with Ruanganshengbaikeli according to 11. 2 and 5. 6 g·kg-1·d-1,group C with Fufangbiejiruanganpian according to 1. 12 g·kg-1·d-1. group D and E with normal isometric normal saline water. The levels of ALT,HA,LN were detected; HGF / c-Met mRNA were tested by RT-PCR. Results The expressions of HGF /c-Met mRNA in group A were more higher than those of other groups,and the liver function,the levels of HA and LN were approaching to those of normal control group,moreover,positive correlation existed among the dosage. Conclusions Ruanganshengbaikeli has therapeutic effect on liver fibrosis,even reverse hepatic cirrhosis to some extent,which might be related to higher expression of HGF / C-Met mRNA in hepatocyte.

关 键 词：软肝升白颗粒 肝纤维化 

分 类 号：R285.5[医药卫生—中药学]