解聚复肾宁对糖尿病大鼠肾组织基质金属蛋白酶-9、金属蛋白酶组织抑制剂-1表达的影响  

Effect of Jiejufushenning on the expressions of MMP-9,TIMP-1 in kidney tissues of diabetes mellitus rats

作　　者：邢磊[1] 焦颖华[2] 田发明[3] 

机构地区：[1]河北联合大学附属医院老年病科,河北唐山063000 [2]河北联合大学附属医院中西医结合科 [3]河北联合大学

出　　处：《中国老年学杂志》2014年第5期1296-1298,共3页Chinese Journal of Gerontology

基　　金：河北省科技计划项目(No.112761138)

摘　　要：目的观察解聚复肾宁(JJFSN)对糖尿病(DM)大鼠肾组织基质金属蛋白酶-9(MMP-9)、金属蛋白酶组织抑制剂-1(TIMP-1)表达及肾脏保护作用机制。方法建立STZ诱导的DM SD大鼠模型,将成模DM大鼠随机分成4组:模型组、JJFSN组、厄贝沙坦组、JJFSN+厄贝沙坦组,同时设正常对照组。各组大鼠采用相应的干预措施处理12 w。检测各组大鼠第12周时肾重/体重、血糖、尿素氮、血肌酐、尿白蛋白排泄率(UAER),免疫组化法检测肾组织MMP-9、TIMP-1表达,透射电镜观察肾脏超微结构。结果模型组肾脏超微结构改变明显,血糖、UAER、尿素氮、血肌酐、肾重/体重显著增高。模型组大鼠肾组织TIMP-1的表达较正常对照组明显上调(P<0.01),JJFSN组、厄贝沙坦组、JJFSN+厄贝沙坦组肾组织TIMP-1表达明显下调(P<0.01),但仍高于正常对照组(P<0.01),JJFSN+厄贝沙坦组下调最明显(P<0.01)。DM模型组大鼠肾组织MMP-9的表达较正常对照组明显下调(P<0.01),解聚复肾宁组、厄贝沙坦组、解聚复肾宁+厄贝沙坦组肾组织MMP-9表达明显上调(P<0.01),但仍低于正常对照组(P<0.01),解聚复肾宁+厄贝沙坦组上调最明显(P<0.01)。结论 MMP-9、TIMP-l的表达变化与肾小球细胞外基质(ECM)降解减少相关,可能促进了糖尿病肾病(DN)的发生,JJFSN可能通过干预这种表达变化,减缓DN的发生和发展。Objective To explore the effect of Jiejufushenning( JJFSN) on expressions of matrix metalloproteinase-9( MMP-9) and tissue inhibitor of matrix metalloproteinases-1( TIMP-1) in diabetic rats and explore the mechanism of JJFSN against renal lesion. Methods The diabetic SD rats were induced by STZ,divided into diabetic control,JJFSN,Irbesartan,JJFSN + Irbesartan and the normal control groups. All rats were respectively treated for 12 weeks by above intervention methods. The relative kidney weight,blood sugar,urea nitrogen,serum creatinine and urinary albumin excretion rate( UAER) were detected by normal methods. Expressions of MMP-9,TIMP-1 in renal tissue were respectively detected by immunohistochemical techniques,and ultramicrostructure of kidney was observed by transmission electron microscope. Results The ultramicrostructure of kidney was noticeably changed,and the blood sugar,UAER,blood urea nitrogen, serum creatinine,and relative kidney weight were significantly increased in diabetic rats. Compared with normal control group,the expression of TIMP-1 was increased in diabetic control group( P < 0. 01). The expression of TIMP-1 in JJFSN,Irbesartan,JJFSN + Irbesartan groups were lower than those in diabetic control group( P<0. 01),but they were still higher than those in normal control group( P<0. 01). The decline in JJFSN + Irbesartan group was most significant( P<0. 01). Compared with normal control group,the expression of MMP-9 was decreased in diabetic control group( P<0. 01). The expressions of MMP-9 in JJFSN,Irbesartan,JJFSN + Irbesartan group were higher than those in diabetic control group( P<0. 01),but they were still lower than those in normal control group( P<0. 01). The raise in JJFSN + Irbesartan group was most significant( P<0. 01). Conclusions The changes of the expressions of MMP-9 and TIMP-1 decrease the degradation extracellular matrix,which promote the occurrence and progress of diabetic nephropathy. JJFSN may attenuate the occurrence and progress of diabetic nephropathy through intervening this

关 键 词：糖尿病 解聚复肾宁 基质金属蛋白酶-9 金属蛋白酶组织抑制剂-1 

分 类 号：R285.5[医药卫生—中药学]