葶苈生脉方对充血性心力衰竭大鼠心肌细胞凋亡相关调控基因的影响  

Effect of Tingli Shengmai decoction on the myocardial apoptosis correlated controlling gene of rats with congestive heart failure

作　　者：苑素云 郭秋红[2] 王卓 苑留云 张一昕 

机构地区：[1]沧州市人民医院,河北沧州061000 [2]河北医科大学中医学院

出　　处：《中国老年学杂志》2014年第4期966-967,共2页Chinese Journal of Gerontology

基　　金：河北省科技攻关计划资助课题(No.0520611034D-9)

摘　　要：目的通过观察不同剂量葶苈生脉方对充血性心力衰竭(CHF)造模大鼠心肌细胞凋亡相关调控基因B细胞淋巴瘤/白血病-2基因(Bcl-2)、促凋亡基因Bax和凋亡蛋白酶Caspase-3表达的影响。方法 60只Wistar大鼠随机分为5组,每组12只。除假手术组外,其余4组手术造成腹主动脉狭窄,假手术组只分离腹主动脉,不结扎。5组均于造模4 w后开始给药。每日1次定量灌胃。5组均连续灌胃8 w。最后1次给药后,取少许心肌组织,运用流式细胞仪检测Bcl-2、Bax和Caspase-3蛋白表达的情况。结果与模型组比较,葶苈生脉方高、低剂量组Bcl-2蛋白表达免疫荧光指数(FI)值明显增加(P<0.05),Bax、Caspase-3蛋白表达FI值和Bax/Bcl-2比值明显降低(P<0.05)。结论葶苈生脉方可通过抑制心肌细胞凋亡,避免减少心肌细胞数量,恢复心肌细胞的功能,达到治疗CHF作用。Objective To explore the mechanism of Tingli Shengmai Decoction's effects by observing the expression of the myocardial apoptosis correlated controlling gene of Bcl-2,Bax and Caspase-3 of rats with congestive heart failure( CHF). Methods 60 male Wistar rats were randomly divided into sham,model,high and low dose of Tingli Shengmai Decoction groups and the Xinbao wan control group. CHF animal models were established by abdominal aortic stenosis method. After common feeding for 4 weeks,all the rats were given intragastric administration once a day and medicine. Expression of gene in myocardial tissue was detected with flow-type cytometry. Results Compared with model group,the expression Bcl-2 of high and low dose groups were significantly increased( P < 0. 05) and the expressions of Bax and Caspase-3 were significantly decreased( P < 0. 05). Conclusions Tingli Shengmai Decoction can treat the CHF by inhibiting the myocardial apoptosis of CHF rats,reducing the loss of myocardial cell during heart failure and recovering the function of myocardial cell.

关 键 词：充血性心力衰竭 中药疗法 细胞凋亡 B细胞淋巴瘤/白血病-2基因 Bax Caspase-3 

分 类 号：R285.5[医药卫生—中药学]