几种常见保存方法对浮游动物生物量的影响  

INFLUENCE OF SEVERAL COMMON PRESERVATION METHODS ON THE BIOMASS OF ZOOPLANKTON

作　　者：冯秋园[1,2] 孙晓霞[1] 任琳琳[1,2] 

机构地区：[1]中国科学院海洋研究所山东胶州湾海洋生态系统国家野外科学观测研究站,青岛266071 [2]中国科学院大学,北京100049

出　　处：《海洋与湖沼》2014年第3期42-48,480,共7页Oceanologia Et Limnologia Sinica

基　　金：中国科学院战略性先导科技专项;XDA05030401号;国家重点基础研究发展计划(973)项目;2014CB441504号;国家海洋公益性行业科研专项;201005014号

摘　　要：5%的甲醛,-20°C冰冻和液氮是常见的浮游动物保存方法。本文以中华哲水蚤(Calanus sinicus)和强壮箭虫(Sagitta crassa)为研究对象,以湿重、干重和碳、氮含量为生物量指标,研究了上述几种保存方法对浮游动物生物量的影响。研究结果显示,对于中华哲水蚤,湿重、干重、碳和氮含量,在用甲醛保存2个月后,分别下降为鲜重的81.03%、14.20%、7.02%和1.13%;冰冻保存2个月后,分别下降为鲜重的66.56%、22.34%、10.30%和2.14%;液氮保存2个月后,分别下降为鲜重的91.85%、26.65%、11.25%和2.41%。对于强壮箭虫,湿重、干重、碳和氮含量,在用甲醛保存2个月后,分别变为鲜重的104%、9%、3.49%和0.69%;冰冻保存2个月后,分别下降为鲜重的73.15%、12.39%、4.82%和1.02%;液氮保存2个月后,分别下降为鲜重的70.72%、11.22%、5.23%和1.13%。在保存初期浮游动物的生物量变化较大,后趋于稳定。甲醛保存方法对浮游动物生物量的影响最大,其次是液氮,冰冻的影响最小。中华哲水蚤的生物量受各种保存方法的影响要小于强壮箭虫。5% formalin,-20°C freeze,and liquid nitrogen are common methods used to preserve zooplankton.Taking Calanus sinicus and Sagitta crassa as target organisms,and wet weight,dry weight,carbon,and nitrogen content as biomass parameters,influences of the above preservation conditions on zooplankton biomass are studied.For Calanus sinicus preserved in formalin for 2 months,the wet weight,dry weight,carbon,and nitrogen content reduced to 81.03%,14.02%,7.02% and 1.13% of the fresh weight,respectively; when frozen at –20°C,they reduced to 66.56%,22.34%,10.30%,and 2.14%,and when preserved in liquid nitrogen,reduced to 91.85%,26.65%,11.25% and 2.42% of the fresh weight,respectively.For Sagitta crassa,the parameters are 104%,9%,3.49% and 0.69% of the fresh weight when preserved in formalin for two month,and 73.15%,12.39%,4.82% and 1.02% when frozen at-20°C,and 70.72%,11.22%,5.23% and 1.13% when preserved in liquid nitrogen,respectively.It was found that the zooplankton biomass decreased quickly at begining,and then tend to stabilize.Preservation in formalin showed the greatest influence on zooplankton biomass,following by in liquid nitrogen and in-20°C freeze.These above three methods of preservation caused smaller impacts on Calanus sinicus than on Sagitta crassa.

关 键 词：生物量 浮游动物 中华哲水蚤 保存方法 保存条件 甲醛溶液 冰冻保存 液氮 保存液 箭虫 

分 类 号：P7[天文地球—海洋科学] P9