基于几何形态测量学的4种金线鱼矢耳石识别研究  

GEOMETRIC MORPHOMETRICS OF SAGITTAL OTOLITH OF FOUR NEMIPTERUS FISH SPECIES

作　　者：侯刚[1,2] 王学锋[1,2] 朱立新 卢伙胜[1,2] 冯波[1,2] 

机构地区：[1]广东海洋大学水产学院,湛江524088 [2]南海渔业资源监测与评估中心,湛江524025 [3]山东大学(威海)海洋学院,威海264209

出　　处：《海洋与湖沼》2014年第3期58-65,496,共8页Oceanologia Et Limnologia Sinica

基　　金：国家自然科学基金资助项目;41006084号;广东省海洋渔业科技推广专项;A201208H01号;广东省自然科学基金资助项目;10152408801000013号;科技部科技支撑项目;SQ2012BAY307301号;农业部南海渔业资源监测专项调查项目(2010-2013)

摘　　要：利用2007年9月至2010年9月采自北部湾海域的24尾红棘金线鱼(Nemipterus nemurus)、32尾金线鱼(N.virgatus)、36尾深水金线鱼(N.bathybius)和35尾日本金线鱼(N.japonicus)性成熟后的矢耳石样本,运用几何形态测量学方法研究了4种鱼类矢耳石形态识别问题。耳石经图像处理后提取地标点坐标值,进行相对扭曲主成分分析,通过判别分析区分4种金线鱼的耳石,利用薄板样条分析和网格变形分析耳石形态变异。结果表明:在相对扭曲主成分分析中,第1、2、3主成分分别解释了总变异的30.78%、28.44%和11.75%,可以将四种金线鱼耳石区分开来。其中Ⅰ型地标点6、10、11,Ⅱ型地标点2,Ⅲ型地标点7,贡献作用较大,解释了主要形态变异。相对扭曲主成分的逐步判别分析中,四种鱼的平均判别成功率达91.3%;交互验证结果显示,四种鱼的平均判别成功率达89.8%,表明几何形态测量学分析可以作为鱼种耳石识别的有效方法之一,在耳石形态学与鱼种判别研究中具有重要应用价值。Identification of sagittal otoliths was investigated in geometric morphometrics among four sympatric species of genus Nemipterus: redspine threadfin bream N.nemurus(n=24),golden threadfin bream N.virgatus(n=32),yellowbelly threadfin bream N.bathybius(n=36),and Japanese threadfin bream N.japonicus(n=35).Specimens were collected in Beibu Gulf from September 2007 to September 2010.Landmark coordinate values were extracted after otolith digitized by processing image using relative warp(RW) as principal component,and grad distortion via thin plate spline.Eighteen RW values were used to differentiate the otoliths among four Nemipterus species.The result indicate that the 1st,2nd and 3rdRWs accounted for 30.78%,28.44%,and 11.75% of the total variation,respectively,among which TypeⅠlandmarks 6,10,and 11,TypeⅡ landmarks 2,and Type Ⅲ landmark 7 are important contributors,and could explain the most variation.Success in identifying the otolith could reach 91.3% by RW-based gradual discrimination,or 89.8% by cross validation,indicating that the geometric morphometrics is a potential tool in distinguishing Nemipterus species with otolith.

关 键 词：金线鱼属 形态测量学 矢耳石 逐步判别分析 主成分分析 标点 形态变异 几何 成功率 识别研究 

分 类 号：P7[天文地球—海洋科学] P9