胎牛视网膜干细胞向神经细胞诱导研究  

Directed Neurons Differentiation of Bovine Retinal Stem Cells

作　　者：孙博[1,2] 张文秀[2] 吴芳春[2] 马月辉[2] 沙伟[1] 关伟军[2] 

机构地区：[1]齐齐哈尔大学生命科学与农林学院,齐齐哈尔161006 [2]中国农业科学院北京畜牧兽医研究所,北京100193

出　　处：《畜牧兽医学报》2014年第4期559-565,共7页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：中国农业科学院科技创新工程(ASTIP-IASO1);国家家养动物种质资源平台项目(2014年)

摘　　要：旨在研究胎牛视网膜干细胞(Retinal stem cells,RSCs)的分离、培养、鉴定及向不同类型细胞诱导分化等的生物学特性。采用机械吹打法从胎牛眼睫状体部细胞中分离RSCs,通过免疫荧光方法鉴定RSCs标志物Nestin、Pax-6和Chx-10,并诱导其向星形胶质细胞、神经元及少突胶质细胞诱导分化,检测其多向分化潜能。结果表明,机械吹打法获得的胎牛RSCs不仅表达干细胞特有的标志,而且成功诱导分化为星形胶质细胞、神经元及少突胶质细胞。综上表明,胎牛RSCs具有良好的体外增殖能力和多向分化潜能。为今后视网膜干细胞的研究提供相应的理论依据。This study aimed to research the biological characteristics of retinal stem cells(RSCs) from bovine fetus.RSCs were isolated using physical method,and the surface markers of RSCs, Nestin,Chx-10and Pax-6,were detected by immunofluorescence.The multi-lineage differentiation potential of RSCs was investigated and induced to differentiate into astrocytes,neurons and oligodendrocytes.The results showed that the fetal bovine RSCs not only expressed the special markers of stem cells but also induced to astrocytes,neurons and oligodendrocytes successfully.To sum up the RSCs isolated from fetal bovine have a high proliferation and multi-directional differentiation potential in vitro.Retinal stem cells for the future research provide the corresponding theoretical foundation.

关 键 词：牛 视网膜干细胞 分化潜能 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]