复方人参皂苷纳米乳的制备及其急性毒性研究  

Study on the Preparation and Acute Toxicity of Nanoemulsion Containing Ginsenoside and Levamisole Hydrochloride

作　　者：曹发昊[1,2] 欧阳五庆[2] 王艳萍[1] 

机构地区：[1]运城学院生命科学系,运城044000 [2]西北农林科技大学动物医学院,杨凌712100

出　　处：《畜牧兽医学报》2014年第4期666-671,共6页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：陕西省重大科技创新专项基金(K332020916);运城学院院级科研项目(CY-2012005)

摘　　要：利用纳米化技术,将人参皂苷和盐酸左旋咪唑组成复方人参皂苷纳米乳,对其组成和急性毒性进行研究。利用伪三元相图筛选出用于制备复方人参皂苷纳米乳的空白纳米乳;研究不同含药量纳米乳对免疫抑制小鼠脾T淋巴细胞增殖的影响,根据免疫增强作用较强的含药纳米乳,确定复方人参皂苷纳米乳的组成;分别用透射电镜、激光粒度仪和小鼠灌胃试验对其形态、粒径和急性毒性进行研究。结果显示,确定的复方人参皂苷纳米乳组成:Solutol?HS-15、甘油、PEG400、IPM、蒸馏水,其质量比为25.33∶10.14∶2.53∶6∶56,GS和LH含量均为30mg·mL-1;它是黄色透明O/W纳米乳,液滴呈球形,平均粒径为23.08nm,粒度分散指数为0.237,小鼠灌胃LD50为402.85mg·kg-1。本试验成功制备了复方人参皂苷纳米乳,为进一步开发纳米化免疫增强剂提供了依据。By using nanotechnology,ginsenoside(GS)and levamisole hydrochloride(LH)were combined with the carrier nanoemulsion to prepare compound GS and LH nanoemulsion(GS-LHNE).Then its composition and acute toxicity were evaluated.The optimum blank nanoemulsion, which was used to prepare GS-LH-NE,was screened out through pseudo-ternary phase diagram. The enhancement effect of nanoemulsion containing different content of drug on the immunologic function of immunosuppressive mice were studied through splenic lymphocytes proliferation test, and the nanoemulsion with a better immunologic enhancement effect was screened out for ascertaining the composition of GS-LH-NE.The morphology of GS-LH-NE was observed using transmission electron microscope;the particle size was measured using laser particle size analyzer;the gavage acute toxicity of GS-LH-NE was measured using mice.The ascertained composition of GSLH-NE was Solutol?HS-15/glycerol/PEG400/IPM/distilled water(with the mass ratio of 25.33: 10.14:2.53:6:56),in which the content of GS and LH were both 30 mg· mL-1 and 30 mg·mL-1,respectively.GS-LH-NE was a yellow transparent oil-in-water nanoemulsion,which had spherical droplets with the particle size of 23.08nm and PDI of 0.237,and its gavage LD50 was 402.85mg·kg-1.GS-LH-NE is successfully prepared and provides the possibility to develop immunologic enhancer through nanotechnology.

关 键 词：人参皂苷 左旋咪唑 纳米乳 免疫增强 

分 类 号：S859.53[农业科学—临床兽医学]