费氏中华根瘤菌042BS(Rhizobium fredii 042BS)蛋白质组学初步研究  

Primary protemic study of Rhizobium fredii 042BS

作　　者：颜廷虎[1] 白庆武[1] 李波[1] 沈世华[2] 

机构地区：[1]齐齐哈尔大学生命科学与工程学院,齐齐哈尔161006 [2]中国科学院植物研究所光合作用与环境分子生理重点研究实验室,北京100093

出　　处：《齐齐哈尔大学学报（自然科学版）》2004年第1期99-101,共3页Journal of Qiqihar University(Natural Science Edition)

摘　　要：为了从蛋白质水平阐明根瘤菌的固氮机理,扩大其宿主植物范围,应用蛋白质组学技术(2-DE),分析比较了能够在大豆和苜蓿上跨宿主结瘤的费氏中华根瘤菌042BS与已经完成基因组测序的根瘤菌模式菌株苜蓿中华根瘤菌(Rhizobium meliloti 1021)的蛋白质表达图谱差异。经过图像分析检测到二者分别有436和428蛋白点。其中约有50个蛋白点初步认为二者所共有的。Since 1994 that Wilkins and Willians clearly coined the concept of proteome, proteomics has stemed out and been used in biology and clinical fields. In our study we compared the 2D-images of R..fredii042BS, which can cross-infect Medicago cativa and soybean, and R..meliloti 1021 served as reference, whose full genome was just sequenced. It was interesting to find there might be 50 protein dots copossessed by those bacteria other than large difference between them in 2D-image.

关 键 词：费氏中华根瘤菌 蛋白质组学 二维凝胶电泳 苜蓿中华根瘤菌 生物固氮 

分 类 号：Q945.13[生物学—植物学]