Study on Storage and Bourgeoning of Spruce Pollen  

Study on Storage and Bourgeoning of Spruce Pollen

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作  者:Ma Jianwei Wang Junhui Zha Shounggong Zhao Qiuling Han Yunhua Zhang Zengshun 

机构地区:[1]Xiaolongshan Research Institute of Forest Science and Technology [2]Key Laboratory of Tree Breeding and Cultivation of State Forestry Administration,Research Institute of Forestry,Chinese Academy of Forestry

出  处:《Chinese Forestry Science and Technology》2012年第3期50-51,共2页中国林业科技(英文版)

摘  要:The effects of sucrose concentration,pH value and storage conditions on germination and pollen tube growth of in vitro cultured spruce were studied and the ingredients of culture medium were optimized.The results showed that the effect of sucrose concentration and pH value on spruce pollen’s germination was significant,and a clear threshold effect existed.Low or high concentration of sucrose was not conducive to the pollen’s germination,it was found that 10%of which was more suitable to the in vitro culture of spruce pollen; different spruce species required different concentrations of sucrose medium,7.5%of sucrose concentration medium was better to pollen tube germination of Picea asperata,but 10%of which was the best medium to that of Picea abies.Both pH 4.5 and 6.5 had restrained action on pollen germinating rate of spruce,and the growth of pollen tubes.Under dark culture conditions,P.abies began to germinate in 6 h,but P.asperata germinated in 8 h,the mass germinating power of P.abies was higher than that of P.asperata;the growth process of P.abies was 20-22 h,and that of P.asperata was about 16 h;The pollen tubes of P.abies was over 10 ?m longer in length than that of P.asperata. There were significant differences of germinating power and length of pollen tubes between genotypes of two different individuals of both spruces.The storage methods had great influence on the viability of pollen.In the process of pollen preservation, temperature was the key factor.Cryopreservation was better than cold storage and room temperature preservation was unfavorable.The effects of sucrose concentration,pH value and storage conditions on germination and pollen tube growth of in vitro cultured spruce were studied and the ingredients of culture medium were optimized.The results showed that the effect of sucrose concentration and pH value on spruce pollen’s germination was significant,and a clear threshold effect existed.Low or high concentration of sucrose was not conducive to the pollen’s germination,it was found that 10%of which was more suitable to the in vitro culture of spruce pollen; different spruce species required different concentrations of sucrose medium,7.5%of sucrose concentration medium was better to pollen tube germination of Picea asperata,but 10%of which was the best medium to that of Picea abies.Both pH 4.5 and 6.5 had restrained action on pollen germinating rate of spruce,and the growth of pollen tubes.Under dark culture conditions,P.abies began to germinate in 6 h,but P.asperata germinated in 8 h,the mass germinating power of P.abies was higher than that of P.asperata;the growth process of P.abies was 20-22 h,and that of P.asperata was about 16 h;The pollen tubes of P.abies was over 10 ?m longer in length than that of P.asperata. There were significant differences of germinating power and length of pollen tubes between genotypes of two different individuals of both spruces.The storage methods had great influence on the viability of pollen.In the process of pollen preservation, temperature was the key factor.Cryopreservation was better than cold storage and room temperature preservation was unfavorable.

关 键 词:POLLEN of SPRUCE VITALITY CULTURE MEDIUM STORAGE method 

分 类 号:S7[农业科学—林学]

 

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