四川省攀西地区南瓜、甜菜和辣椒部分病毒种类的ELISA检测  

ELISA Detection for Certain Virus Types of Squash,Beet and Chili in Panxi District of Sichuan Province

作　　者：杨会房[1] 阮涛[1] 包凌云[1] 于云奇[1] 杨水英[1] 青玲[1] 

机构地区：[1]西南大学植物保护学院植物病害生物学重庆市高校级重点实验室,重庆400716

出　　处：《中国蔬菜》2011年第16期91-94,共4页China Vegetables

基　　金：国家自然科学基金项目(30971897)

摘　　要：利用马铃薯Y病毒(Potato virus Y,PVY)和马铃薯X病毒(Potato virus X,PVX)的多克隆抗体,以及烟草花叶病毒(Tobacco mosaic virus,TMV)、黄瓜花叶病毒(Cucumber mosaic virus,CMV)、蚕豆萎蔫病毒2号(Broad bean wilt virus 2,BBWV-2)及芜菁花叶病毒(Turnip mosaic virus,TuMV)的单克隆抗体,采用抗原直接包被ELISA法对四川省攀枝花、西昌和德昌地区的南瓜、甜菜和辣椒上采集的病毒病样品进行了检测。结果表明,在87份样品中PVY的侵染最普遍,总检出率达74.71%;CMV、TMV、TuMV、BBWV-2和PVX的总检出率分别为12.64%、13.79%、22.99%、10.34%和14.94%。在87份样品中,仅发现TuMV、PVX及PVY的单独侵染;其余25份样品均检测到2种以上病毒的复合侵染,总检出率为34.72%。表明攀西地区蔬菜上病毒复合侵染现象普遍,PVY是该地区蔬菜上的优势毒原。The squash,beet and chili plants with viral symptoms,collected from Panzhihua,Xichang and Dechang Districts of Sichuan Province,were detected by antigen-coated plate(ACP)-ELISA using polyclonal antibodies against Potato virus Y(PVY)and Potato virus X(PVX),and monoclonal antibodies against Tobacco mosaic virus(TMV),Cucumber mosaic virus(CMV),Broad bean wilt virus 2(BBWV-2)and Turnip mosaic virus(TuMV).The results showed that the infection percentage(74.71 %)of PVY was the highest in total 87 samples.12.64 %,13.79 %,22.99 %,10.34 % and 14.94 % samples were infected by CMV,TMV,TuMV,BBWV-2 and PVX,respectively.Other 25 samples(34.72 %)were mix-infected with more than 2 viruses.These results indicated that mix-infections of viruses occurred seriously and PVY was presently dominant virus on vegetables in Panxi District.

关 键 词：南瓜 甜菜 辣椒 病毒病 ELISA检测 

分 类 号：S432.4[农业科学—植物病理学]