New bienzymatic strategy for glucose determination by immobilized-gold nanoparticle-enhanced chemiluminescence  

作　　者：LIN JieHua ZHANG Hui ZHANG ShuSheng 

机构地区：[1]Key Laboratory of Eco-chemical Engineering,Ministry of Education,College of Chemistry and Molecular Engineering,Qingdao University of Science and Technology,Qingdao 266042,China

出　　处：《Science China Chemistry》2009年第2期196-202,共7页中国科学（化学英文版）

基　　金：Supported by the Natural Science Foundation of Shandong Province (Grant No. Q2007B03);the Doctoral Fund of Qingdao University of Science and Technology (Grant No. 0022141);the National Natural Science Foundation of China (Grant No. 20775038)

摘　　要：Bienzymatic biosensor for the determination of glucose by flow injection chemiluminescence(CL) de-tection was proposed.Hybrids of gold nanoparticles(GNPs) and chitosan were chosen as the immobi-lization matrix of glucose oxidase(GOD) and horseradish peroxidase(HRP) to fabricate the biosensors with silane-pretreated glass microbeads.After the enzyme catalyzing oxidation of glucose in GOD biosensor,the produced H2O2 flowed into HRP biosensor to react with luminol.The doped GNPs in chitosan were found to enhance the classical CL reaction of luminol-H2O2-HRP.The CL enhancement was investigated in detail by CL and UV-visible spectrum.Under the optimized experimental conditions,glucose could be determined in a linear range from 0.01 to 6.0 mmol/L with a detection limit of 5.0 μmol/L at 3σ.The accuracy of the proposed method was examined by detecting the glucose level in four clinical serum samples from hospital.The proposed method provides a new alternative to deter-mine glucose.Bienzymatic biosensor for the determination of glucose by flow injection chemiluminescence (CL) detection was proposed. Hybrids of gold nanoparticles (GNPs) and chitosan were chosen as the immobilization matrix of glucose oxidase (GOD) and horseradish peroxidase (HRP) to fabricate the biosensors with silane-pretreated glass microbeads. After the enzyme catalyzing oxidation of glucose in GOD biosensor, the produced H2O2 flowed into HRP biosensor to react with luminol. The doped GNPs in chitosan were found to enhance the classical CL reaction of luminol-H2O2-HRP. The CL enhancement was investigated in detail by CL and UV-visible spectrum. Under the optimized experimental conditions, glucose could be determined in a linear range from 0.01 to 6.0 mmol/L with a detection limit of 5.0 μmol/L at 3σ. The accuracy of the proposed method was examined by detecting the glucose level in four clinical serum samples from hospital. The proposed method provides a new alternative to determine glucose.

关 键 词：CHEMILUMINESCENCE biosensor GLUCOSE gold nanoparticles HORSERADISH PEROXIDASE GLUCOSE OXIDASE 

分 类 号：R446.1[医药卫生—诊断学]