Development of an amperometric glucose biosensor based on the immobilization of glucose oxidase in an ormosil-PVA matrix onto a Prussian Blue modified electrode  

作　　者：HaiLing Chen  Li Zhao  Xi Chen  ZhiXia Zhuang XiaoRu Wang 

机构地区：[1] Testing Center for Food and Drug Safety, Huaxia Vocational College, Xiamen, 361005, China [2] State Key Laboratory of Marine Environmental Science, Xiamen University, Xiamen, 361005, China

出　　处：《Science China Chemistry》2009年第8期1128-1135,共8页中国科学（化学英文版）

基　　金：Supported by the National High Technical Development Project (863 project) Foundation (Grant No. 2006AA09Z160);the National Natural Science Foundation of China (Grant No. 20775064)

摘　　要：An amperometric glucose biosensor was developed based on the immobilization of glucose oxidase in the organically modified silicate (ormosil)-polyvinyl acetate (PVA) matrix onto a Prussian Blue (PB)-modified glassy carbon electrode. A higher stability PB-modified electrode was prepared by the electrochemical deposition of FeCl<SUB>3</SUB>, K<SUB>3</SUB>[Fe(CN)<SUB>6</SUB>] and ethylenediamine tetraacetic acid (EDTA) under cyclic voltammetric (CV) conditions. The effects of the potential range of CV conditions, electrolyte cations, applied potential, pH, temperature and co-existing substances were investigated. The detection limit of the glucose biosensor was 8.1 &#956;mol·L<SUP>&#8722;1</SUP> (S/N = 3) with a linear range from 20 &#956;mol·L<SUP>&#8722;1</SUP> to 2 mmol·L<SUP>&#8722;1</SUP> (R = 0.9965). The biosensor presented a fast response and good selectivity. Additionally, excellent reproducibility and stability of the biosensor were observed.An amperometric glucose biosensor was developed based on the immobilization of glucose oxidase in the organically modified silicate(ormosil)-polyvinyl acetate(PVA) matrix onto a Prussian Blue(PB)-modified glassy carbon electrode.A higher stability PB-modified electrode was prepared by the electrochemical deposition of FeCl3,K3[Fe(CN)6] and ethylenediamine tetraacetic acid(EDTA) under cyclic voltammetric(CV) conditions.The effects of the potential range of CV conditions,electrolyte cations,applied potential,pH,temperature and co-existing substances were investigated.The detection limit of the glucose biosensor was 8.1 μmol·L-1(S/N = 3) with a linear range from 20 μmol·L-1 to 2 mmol·L-1(R = 0.9965).The biosensor presented a fast response and good selectivity.Additionally,excellent reproducibility and stability of the biosensor were observed.

关 键 词：Prussian Blue glucose OXIDASE ormosil-PVA ETHYLENEDIAMINE tetraacetic acid biosensor 

分 类 号：TP212.3[自动化与计算机技术—检测技术与自动化装置]