Sulfonate-based fluorescent probes for imaging hydrogen peroxide in living cells  

作　　者：XU KeHua LIU Fen WANG HuiXia WANG ShanShan WANG LuLu TANG Bo 

机构地区：[1]Engineering Research Center of Pesticide and Medicine Intermediate Clean Production,Ministry of Education,and Key Laboratory of Molecular and Nano Probes,Ministry of Education,College of Chemistry,Chemical Engineering and Materials Science,Shan-dong Normal University,Jinan 250014,China

出　　处：《Science China Chemistry》2009年第6期734-740,共7页中国科学（化学英文版）

基　　金：Supported by the National Basic Research Program of China (Grant No. 2007CB936000);the National Natural Science Funds for Distinguished Young Scholar (Grant No. 20725518);Major Program of the National Natural Science Foundation of China (Grant No. 90713019);the National Natural Science Founda-tion of China (Grant No. 20875057);the Natural Science Foundation of Shandong Province, China (Grant No. Y2007B02);the Science and Technology Develop-ment Programs of Shandong Province, China (Grant No. 2008GG30003012)

摘　　要：Based on the mechanism of H2O2-mediated hydrolysis of sulfonates, two fluorescein disulfonates compounds (FS-1 and FS-2) were designed and synthesized as the highly selective and sensitive fluorescent probes for imaging H2O2 in living cells. The probes were detected with elemental analysis, IR, 1H NMR and 13C NMR. Upon reaction with H2O2, the probes exhibit strong fluorescence responses and high selectivity for H2O2 over other reactive oxygen species and some biological compounds. Furthermore, the sulfonate-based probes, as novel fluorescent reagents, are cell-permeable and can detect micromolar changes in H2O2 concentrations in living cells by using confocal microscopy.Based on the mechanism of H2O2-mediated hydrolysis of sulfonates, two fluorescein disulfonates compounds (FS-1 and FS-2) were designed and synthesized as the highly selective and sensitive fluorescent probes for imaging H2O2 in living cells. The probes were detected with elemental analysis, IR, 1H NMR and 13C NMR. Upon reaction with H2O2, the probes exhibit strong fluorescence responses and high selectivity for H2O2 over other reactive oxygen species and some biological compounds. Furthermore, the sulfonate-based probes, as novel fluorescent reagents, are cell-permeable and can detect micromolar changes in H2O2 concentrations in living cells by using confocal microscopy.

关 键 词：FLUORESCEIN disulfonates FLUORESCENT probe cell IMAGING hydrogen PEROXIDE 

分 类 号：Q2-3[生物学—细胞生物学]