Electrochemical sensing of ATP with synthetic cyclophane as recognition element  

作　　者：MA YuRong ZHAO Hong YU Ping SU Lei ZHANG DeQing MAO LanQun 

机构地区：[1]Graduate University of Chinese Academy of Sciences,Beijing 100049,China [2]Beijing National Laboratory for Molecular Sciences,Institute of Chemistry,Chinese Academy of Sciences,Beijing 100190,China

出　　处：《Science China Chemistry》2009年第6期741-745,共5页中国科学（化学英文版）

基　　金：Supported by the National Natural Science Foundation of China (Grant Nos. 20625515, 90813032, 20721140650 & 20705034);National Basic Research Program of China (Grant No. 2007CB935603);President Fund of GUCAS (Grant No. 065001BY00)

摘　　要：A new electrochemical sensor for ATP with synthetic cyclophane stably attached onto single-walled carbon nanotubes (SWNTs) as the recognition elements is described. UV-vis and cyclic voltammetric results demonstrate that ATP may interact with the synthetic cyclophane recognition elements to form a stable adduct mainly through electrostatic, π-π stacking and donor-acceptor interactions. Such interactions eventually lead to a decrease in the peak currents of the cyclophane recognition elements attached onto the SWNT electronic transducer, which could be used for electrochemical sensing of ATP. Under the conditions employed here, the ratio of the decrease in the anodic peak current is linear with ATP concentration within a concentration range from 10 to 120 μM with a linear coefficiency of 0.993. This study may offer a new and simple electrochemical approach for effective sensing of ATP.A new electrochemical sensor for ATP with synthetic cyclophane stably attached onto single-walled carbon nanotubes (SWNTs) as the recognition elements is described. UV-vis and cyclic voltammetric results demonstrate that ATP may interact with the synthetic cyclophane recognition elements to form a stable adduct mainly through electrostatic, π-π stacking and donor-acceptor interactions. Such interactions eventually lead to a decrease in the peak currents of the cyclophane recognition elements attached onto the SWNT electronic transducer, which could be used for electrochemical sensing of ATP. Under the conditions employed here, the ratio of the decrease in the anodic peak current is linear with ATP concentration within a concentration range from 10 to 120 μM with a linear coefficiency of 0.993. This study may offer a new and simple electrochemical approach for effective sensing of ATP.

关 键 词：ELECTROCHEMISTRY sensor ATP clyclophane 

分 类 号：Q50[生物学—生物化学]