Expression pattern of antibacterial genes in the Musca domestica  

作　　者：WANG Yan1,JIN XiaoBao1,ZHU JiaYong1,ZENG AiHua2,CHU FuJiang1,YANG XiaoRong1 & MA Yan1 1 Institute of Pathogen Biology and Department of Pathogen Biology,Guangdong Pharmaceutical University,Guangzhou 510006,China 2 Department of Pharmacology,Guangdong Pharmaceutical University,Guangzhou 510006,China 

出　　处：《Science China(Life Sciences)》2009年第9期823-830,共8页中国科学（生命科学英文版）

基　　金：Supported by the National Natural Science Foundation of China (Grant No. 30671832);the Key Science and Technology Program of Guangzhou (Grant No. 2005Z32E0211)

摘　　要：This work studied the transcriptional patterns of three antibacterial genes,attacin,defensin and cecropin,during the development of Musca domestica. Quantitative analysis by real-time PCR was performed on mRNA levels in different development stages and challenged 3rd-instar larva at different time points after challenge of Musca domestica. The results revealed a predominance of the transcripts of all three genes during the 3rd-instar larvae and the adults. In the meanwhile,it revealed the greatest increase in mRNA. The transcript levels increased to 801 times,1009 times and 2500 times respectively for cecropin,attacin and defensin in 3rd-instar larvae after challenging susceptible bacterium. The results suggested that the transcriptional patterns of Musca domestica antibacterial genes were different during the different growth stages as well as the microbial challenge encountered in 3rd-instar larvae.This work studied the transcriptional patterns of three antibacterial genes,attacin,defensin and cecropin,during the development of Musca domestica. Quantitative analysis by real-time PCR was performed on mRNA levels in different development stages and challenged 3rd-instar larva at different time points after challenge of Musca domestica. The results revealed a predominance of the transcripts of all three genes during the 3rd-instar larvae and the adults. In the meanwhile,it revealed the greatest increase in mRNA. The transcript levels increased to 801 times,1009 times and 2500 times respectively for cecropin,attacin and defensin in 3rd-instar larvae after challenging susceptible bacterium. The results suggested that the transcriptional patterns of Musca domestica antibacterial genes were different during the different growth stages as well as the microbial challenge encountered in 3rd-instar larvae.

关 键 词：Musca domestica expression pattern ATTACIN DEFENSIN CECROPIN 

分 类 号：Q78[生物学—分子生物学]