HIV-specific CD8^+ T cell responses to HXB2 Gag and Nef peptide pools in Chinese HIV/AIDS patients  

作　　者：ZHANG HongWei,QIU ZhiFeng,JIAO Yang,WANG AiXia & LI TaiSheng Department of Infectious Diseases,Peking Union Medical College Hospital,Chinese Academy of Medical Sciences,Beijing 100730,China 

出　　处：《Science China(Life Sciences)》2009年第9期841-846,共6页中国科学（生命科学英文版）

基　　金：Supported by the National Key High-technology R&D Programm for the 11th Five-year Plan (Grant No. 2008ZX10001-006);the Ministry of Health Clinical HIV/AIDS Research Grant (Grant No. 2007-2009);the Therapeutic Drug Monitoring of Anti-HIV Regimens and HIV Resistance Testing in Beijing HIV/AIDS Patients Receiving HAART,Beijing Science and Technology Program Fund (Grant No. D0906003040491)

摘　　要：HXB2 is primarily used as a template strain in developing HIV vaccines in Europe and the US. However,it is not yet known whether the strain can induce strong HIV-specific CD8+ T cell responses in Chinese HIV/AIDS patients. In the present study,two groups of subjects were investigated:9 AIDS patients and 7 long-term nonprogressors (LTNPs). HIV-specific CD8+ T cell responses were examined in all patients through the ELISPOT assay. CD4+ T cell counts,CD8+ T cell counts,viral load and HIV subtype of each patient were also measured. Thailand B virus strain was identified among all the patients. The breadth and magnitude of HIV-specific CD8+ T cell responses in the LTNPs group are greater than those in the AIDS group (P<0.01). There is a positive correlation between magnitude of HIV-specific CD8+ T cell responses and CD4+ T cells,and a negative correlation between HIV-specific CD8+ T cell responses and mean viral load. In summary,the HIV-specific CD8+ T cell responses to the HXB2 Gag and Nef peptide pools are considerable in Chinese HIV/AIDS patients infected with Thailand B virus strain. HIV-1 vaccines based on HXB2 strain that can induce extensive immunity may be helpful for Chinese.HXB2 is primarily used as a template strain in developing HIV vaccines in Europe and the US. However,it is not yet known whether the strain can induce strong HIV-specific CD8+ T cell responses in Chinese HIV/AIDS patients. In the present study,two groups of subjects were investigated:9 AIDS patients and 7 long-term nonprogressors (LTNPs). HIV-specific CD8+ T cell responses were examined in all patients through the ELISPOT assay. CD4+ T cell counts,CD8+ T cell counts,viral load and HIV subtype of each patient were also measured. Thailand B virus strain was identified among all the patients. The breadth and magnitude of HIV-specific CD8+ T cell responses in the LTNPs group are greater than those in the AIDS group (P<0.01). There is a positive correlation between magnitude of HIV-specific CD8+ T cell responses and CD4+ T cells,and a negative correlation between HIV-specific CD8+ T cell responses and mean viral load. In summary,the HIV-specific CD8+ T cell responses to the HXB2 Gag and Nef peptide pools are considerable in Chinese HIV/AIDS patients infected with Thailand B virus strain. HIV-1 vaccines based on HXB2 strain that can induce extensive immunity may be helpful for Chinese.

关 键 词：HIV/AIDS CD8+ T cells ELISPOT assay 

分 类 号：R512.91[医药卫生—内科学]