Recent progress in quantitative analysis of DNA adducts of nephrotoxin aristolochic acid  

作　　者：YUE Hao CHAN Wan YU KongJie GUO Lin LIU Ning LIU HuWei CAI ZongWei 

机构地区：[1]Department of Chemistry,Hong Kong Baptist University,Hong Kong SAR,China [2]Changchun University of Chinese Medicine,Changchun 130117,China [3]Fujian Entry-Exit Inspection and Quarantine Bureau,Fuzhou 350001,China [4]Institute of Analytical Chemistry,College of Chemistry and Molecular Engineering,Peking University,Beijing 100871,China

出　　处：《Science China Chemistry》2009年第10期1576-1582,共7页中国科学（化学英文版）

基　　金：Supported by the Research Grant Council,University Grants Committee of Hong Kong (Grant No. HKBU2459/06M);the Health and Health Services Research Fund of Hong Kong (Grant No. 05060141)

摘　　要：Aristolochic acid (AA), a mixture of structure-related nitrophenanthrene carboxylic acid derivatives derived from Aristolochia spp, is associated with nephrotoxin and carcinogen. AA-DNA adducts induced by reductive metabolic activation of AA were detected in tissues of animals and in patients exposed to AA. The DNA adducts were generally used as biomarkers in toxicological study of AA. In this short review, quantitative analysis of AA-DNA adducts in various in vitro and in vivo systems by using 32P-postlabelling assay, HPLC-UV, HPLC-radiation monitor, HPLC-FLD, HPLC-ESI/MS and UPLC-MS/MS methods is discussed. The distribution of AA-DNA adducts in various tissues is also summarized.Aristolochic acid (AA), a mixture of structure-related nitrophenanthrene carboxylic acid derivatives derived from Aristolochia spp, is associated with nephrotoxin and carcinogen. AA-DNA adducts induced by reductive metabolic activation of AA were detected in tissues of animals and in patients exposed to AA. The DNA adducts were generally used as biomarkers in toxicological study of AA. In this short review, quantitative analysis of AA-DNA adducts in various in vitro and in vivo systems by using 32P-postlabelling assay, HPLC-UV, HPLC-radiation monitor, HPLC-FLD, HPLC-ESI/MS and UPLC-MS/MS methods is discussed. The distribution of AA-DNA adducts in various tissues is also summarized.

关 键 词：aristolochic ACID DNA ADDUCTS QUANTITATIVE analysis SHORT REVIEW 

分 类 号：Q355[生物学—遗传学]