Construction of EGFP-tagged rBCG of E.tenella and distribution in chickens  

作　　者：WANG QiuYue1,LI JianHua1,ZHANG XiChen1,LIU ChengWu2,CAO LiLi1,REN KeYan3,GONG PengTao1,CAI YaNan11 College of Animal Science and Veterinary Medicine,Jilin University,Changchun 130062,China 2 Shenyang Police Dog Training School,Shenyang 110034,China 3 Veterinary Science Institute of Jilin Province,Changchun 130062,China 

出　　处：《Science China(Life Sciences)》2009年第3期278-283,共6页中国科学（生命科学英文版）

基　　金：Supported by Grants from the Foundation of the Jilin Provincial Science and Technology Department of China (Grant No.20050211-2);the National Natural Science Foundation of China (Grant No.30170696,30500370 and 30671580)

摘　　要：Chicken coccidiosis is a major parasitic disease with substantial economic burden to the poultry industry.Enhanced green fluorescent protein(EGFP) tagged recombinant Bacille Calmette-Guerin(rBCG),as a fusion protein with coccidian rhomboid antigen was constructed to track rBCG in vivo in chickens in this study.Immunization of chickens with one dose of rBCG pMV361-Rho/EGFP induced humoral immune response.The colonization of rBCG in liver,spleen,lung,kidney and caecum was observed by laser confocal microscopy.Real-time quantitative RT-PCR showed a rise expression level of rhomboid protein on the 7th day and a peak on the 14th day and disappearance on the 28th day after immunization.These results have significant implications for the development of rBCG vaccines against avian coccidiosis.Chicken coccidiosis is a major parasitic disease with substantial economic burden to the poultry industry.Enhanced green fluorescent protein(EGFP) tagged recombinant Bacille Calmette-Guerin(rBCG),as a fusion protein with coccidian rhomboid antigen was constructed to track rBCG in vivo in chickens in this study.Immunization of chickens with one dose of rBCG pMV361-Rho/EGFP induced humoral immune response.The colonization of rBCG in liver,spleen,lung,kidney and caecum was observed by laser confocal microscopy.Real-time quantitative RT-PCR showed a rise expression level of rhomboid protein on the 7th day and a peak on the 14th day and disappearance on the 28th day after immunization.These results have significant implications for the development of rBCG vaccines against avian coccidiosis.

关 键 词：COCCIDIOSIS rBCG EGFP RHOMBOID laser CONFOCAL microscopy real-time RT-PCR 

分 类 号：S831[农业科学—畜牧学]