Synthesis, DNA-binding and photocleavage studies of ruthenium(Ⅱ) complexes [Ru(btz)_3]^(2+) and [Ru(btz)(dppz)_2]^(2+)  

作　　者：YU HuiJuan, HUANG ShuMei, KOU JunFeng, LI LvYing, JIA HaiNa, CHAO Hui & JI LiangNian MOE Laboratory of Bioinorganic and Synthetic Chemistry, School of Chemistry and Chemical Engineering, Sun Yat-Sen University, Guangzhou 510275, China 

出　　处：《Science China Chemistry》2009年第9期1504-1511,共8页中国科学（化学英文版）

基　　金：Supported by the 973 Program of China (Grant No 2007CB815306);the National Natural Science Foundation of China (Grant Nos 20771105 & 20871122);the Pro-gram for New Century Excellent Talents in University (Grant No NCET-06-0718);the Key Project (Grant No 108103) of Ministry of Education;the Natural Sci-ence Foundation of Guangclong Province (Grant No 9351027501000003)

摘　　要：Two new ruthenium(Ⅱ) complexes, [Ru(btz)3](ClO4)2 (1) and [Ru(btz)(dppz)2](ClO4)2 (2) (btz = 4,4′-bithi-azole, dppz = dipyrido[3,2-a:2′,3′-c]phenazine), have been synthesized and characterized by elemental analysis, 1H NMR, ES-MS and X-ray crystallography. The DNA binding behaviors of two complexes have been studied by spectroscopic and viscosity measurements. The results suggest that complex 1 binds to CT-DNA via an electrostatic mode, while complex 2 via an intercalative mode. Under irradiation at 365 nm, both complexes were found to promote the cleavage of plasmid pBR 322 DNA from supercoiled form Ⅰ to nicked form Ⅱ. The mechanism studies reveal that singlet oxygen 1O2 and hydroxyl radical (OH-) play a significant role in the photocleavage process.Two new ruthenium(Ⅱ) complexes, [Ru(btz)3](ClO4)2 (1) and [Ru(btz)(dppz)2](ClO4)2 (2) (btz = 4,4′-bithi-azole, dppz = dipyrido[3,2-a:2′,3′-c]phenazine), have been synthesized and characterized by elemental analysis, 1H NMR, ES-MS and X-ray crystallography. The DNA binding behaviors of two complexes have been studied by spectroscopic and viscosity measurements. The results suggest that complex 1 binds to CT-DNA via an electrostatic mode, while complex 2 via an intercalative mode. Under irradiation at 365 nm, both complexes were found to promote the cleavage of plasmid pBR 322 DNA from supercoiled form Ⅰ to nicked form Ⅱ. The mechanism studies reveal that singlet oxygen 1O2 and hydroxyl radical (OH-) play a significant role in the photocleavage process.

关 键 词：ruthenium(Ⅱ) complex DNA binding DNA PHOTOCLEAVAGE PDT 

分 类 号：O621.1[理学—有机化学]