甲基对硫磷分子印迹整体柱的制备及其在固相萃取中的应用  

Preparation of methyl parathion imprinted Polymer and Its Application for Solid-phase Extraction

作　　者：杨淑玲[1] 郑红[1] 程子毓[1] 杲秀英 

机构地区：[1]青海师范大学化学系,青海西宁810008

出　　处：《青海师范大学学报（自然科学版）》2009年第4期47-50,共4页Journal of Qinghai Normal University(Natural Science Edition)

基　　金：教育部科学技术研究重点项目(2008155)资助;青海省科技厅项目(2008-G-166)

摘　　要：以甲基对硫磷为模板分子,采用原位逐步聚合法制备了具有良好识别性能的分子印迹聚合物(MIP)整体柱,系统考察了甲基对硫磷与其结构类似物在该柱上的选择性分离富集特性.用聚合物整体柱固相萃取了蜂蜜中的甲基对硫磷.结果表明,聚合物整体柱对模板分子产生了印迹效应,对甲基对硫磷有明显的选择性.本文以采用C18色谱柱,以甲醇-水(体积比为85:15)为流动相,流速为1.0mL/min,吸收波长为270nm,可在15分钟实现甲基对硫磷的测定.A molecularly imprinted solid phase extractant,particularly for the recognition of methyl parathion was prepared using methyl parathion as the template and in situ step-addition polymerization.The characteristics of selective separation and preconcentration of methyl parathion and its structural analogs on the column were examined.Using polymer solid-phase extract the methyl parathion in the honey.The results indicated that different kinds of recognition sites in the MIP and the MIP showed specific affinity toward methyl parathion.Using C18 reversed-phase column,mobile phase as methanol-water(volume ratio of 85:15),the velocity of flow was 1.0 ml/min,detection wavelength was at 270 nm.

关 键 词：分子印迹聚合物 整体柱 甲基对硫磷 

分 类 号：O657.7[理学—分析化学]