四种副溶血弧菌总RNA提取方法的比较  

Comparison of four Kinds of Methods for Extracting Total RNA from Vibrio parahaemolyticus

作　　者：陈星[1] 潘迎捷[1] 孙晓红[1] 赵勇[1] 

机构地区：[1]上海海洋大学食品学院,上海201306

出　　处：《基因组学与应用生物学》2009年第6期1177-1182,共6页Genomics and Applied Biology

基　　金：863计划重点项目(2008AA100804);上海市科委重大科技攻关项目(07dz19508);霍英东教育基金会第十一届高等院校青年教师优选资助课题(114035);"十一五"国家科技支撑计划课题--世博科技专项;世博猪肉产品安全优质供给体系研究及产业化示范(2009BAK43B17)共同资助

摘　　要：副溶血弧菌(Vibrio parahaemolyticus)是一种革兰氏阴性嗜盐菌。提取高质量的RNA是研究副溶血弧毒力基因表达与其致病性和环境适应性的基础。本研究分别用SDS法、Trizol法,PureLinkTM RNA Mini Kit和Uniq-10柱式Trizol总RNA抽提试剂盒提取六株副溶血弧菌的总RNA,用普通琼脂糖凝胶电泳和核酸测定仪检测RNA的质量,并用RT-PCR法对四种提取方法进行比较。研究结果表明,Trizol法和Pure-LinkTM RNA Mini Kit简单快捷,提取的总RNA完整性好,纯度高,可用于后续实验的分子生物学研究;而Trizol法更适用于普通实验室细菌RNA的提取。Vibrio parahaemolyticus is a kind of halophilic gram-positive bacterias.Extracting high-quality total RNA from Vibrio parahae molyticus is essential for studying virulence gene expression and its pathogenicity and environmental adaptability.In this research,we extracted total RNA from six strains by using SDS method,Trizol method,PureLinkTM RNA Mini Kit and Uniq-10 column Trizol total RNA extraction kit.Then detected the quality of extracted RNA with ordinary agarose gel electro phoresis and nucleic acid detector,and comparison of four kinds of extraction methods by using RT-PCR method.The results showed that Trizol method and PureLinkTM RNA Mini Kit are simple and rapid,which total RNA isolated byabove two methods has the characteristic of good integrity and high purity,and can be used for subsequent molecular biology research.However,Trizol method approriated for isolating total RNA of bacterial from ordinary laboratory.

关 键 词：副溶血弧菌 总RNA提取 SDS法 TRIZOL法 试剂盒 

分 类 号：Q933[生物学—微生物学]