基因表达系列分析法(SAGE)的改进及其在植物功能基因组研究中的应用  

The Modification of Serial Analysis of Gene Expression(SAGE) and its Application in Plant Functional Genome Research

作　　者：张振乾[1] 谭太龙[1] 肖钢[1] 官春云[1] 

机构地区：[1]湖南农业大学农学院,国家油料改良中心湖南分中心,长沙410128

出　　处：《基因组学与应用生物学》2009年第6期1204-1210,共7页Genomics and Applied Biology

基　　金：国家973计划(2006CB101600)资助

摘　　要：基因表达系列分析方法(SAGE)是一种新的基因表达分析方法,与基因芯片技术一样具有高通量的特点,可测定特定组织的基因表达水平,在全基因组水平上同时定量检测数万个基因表达模式;可在未知目的基因的前提下,分析来自一个细胞的全部转录本信息;对已知或未知基因表达进行定性和定量分析。目前,虽然在疾病、发育、细胞凋亡、药物筛选等多个领域已有利用SAGE方法进行的研究,但该方法在植物功能基因组研究中的应用相对较少。本文主要综述了该方法在RNA用量、PCR循环次数、SAGE效能和可靠性、标签长度和未知标签分析等方面的改进及其在植物中构建SAGE文库、筛选新基因、基因表达图谱分析等方面的应用,从而为其在植物功能基因组研究中的进一步应用提供理论参考。Serial analysis of gene expossion(SAGE) is a kind of innovative gene expossion analysis method,which possesses the same characteristic of high throughput as Genechip.This techniques can determine the level of gene expression in some special tissues and can quantitatively detect expression patterns of thousands of genes simultaneously on the whole genome level.And it also can be used to analyze all transcripts information from a cell without knowing target gene.Furthermore,This analytical method can carry out the qualitative and quantitative analysis for the expression of known or unknown genes.At present,SAGE has been widely used in many fields,such as disease,development,apoptosis and drug screening etc.,whereas it is relatively less been applied in plant functional genome research.In this paper,we mainly reviewed the modification of this approach on RNA dosage,PCR cycles,the efficacy and reliability of SAGE,the label length and unknown label analysis and so on.At the same time,we also summarized its application in plant functional genome research,for instance,the construction of SAGE library,screening of new genes and gene expression profiling analysis,which would provide theory reference for its further application in plant functional genome research.

关 键 词：基因系列分析方法(SAGE) 功能基因组 基因表达图谱分析 

分 类 号：Q943.2[生物学—植物学]