Adsorption equilibrium of binary proteins to Q Sepharose FF  

作　　者：LIANG Juan~(1,2),FIEG Georg~2,LUO Xing~2,SHI Qinghong~1,SUN Yan~1 (1 Department of Biochemical Engineering and Key Laboratory of Systems Bioengineering of Ministry of Education, School of Chemical Engineering and Technology,Tianjin University,Tianjin 300072,China 2 Institute of Process and Plant Engineering,Hamburg University of Technology,Schwarzenbergstrasse 95,21073 Hamburg,Germany) 

出　　处：《化工进展》2011年第S2期190-208,共19页Chemical Industry and Engineering Progress

基　　金：Supported by the Natural Science Foundation of China(No.20636040 and 20976126);Natural Science Foundation of Tianjin from Tianjin Municipal Science and Technology Commission(09JCYBJC06800);the Program for New Century Excellent Talents in University from the Ministry of Education of China(NCET-08-0387)

摘　　要：Simultaneous and sequential adsorptions of bovine serum albumin(BSA) and bovine hemoglobin(bHb) was investigated on anion ion-exchange media Q Sepharose FF in batch adsorption experiments to elucidate the mechanism of multi-component protein adsorption.BSA is always preferential adsorbed with bHb inhibited in binary adsorption.Influence on binary adsorption of factors such as pH,salt concentration,initial concentration ratios of proteins and the adsorption sequence are determined.Three models have been tested and compared to describe the competitive binary adsorption of BSA and bHb in all conditions studied.Among the extended Langmuir model gives the best predict of adsorption of the stronger adsorbed protein when its adsorption is not very strong in simultaneous adsorption,the Statistical thermodynamic(ST) model gives the best description of weaker adsorbed protein in simultaneous adsorption,the Steric mass-action(SMA) model is most appropriate multi-component model considering all different conditions for both proteins.Simultaneous and sequential adsorptions of bovine serum albumin(BSA) and bovine hemoglobin(bHb) was investigated on anion ion-exchange media Q Sepharose FF in batch adsorption experiments to elucidate the mechanism of multi-component protein adsorption.BSA is always preferential adsorbed with bHb inhibited in binary adsorption.Influence on binary adsorption of factors such as pH,salt concentration,initial concentration ratios of proteins and the adsorption sequence are determined.Three models have been tested and compared to describe the competitive binary adsorption of BSA and bHb in all conditions studied.Among the extended Langmuir model gives the best predict of adsorption of the stronger adsorbed protein when its adsorption is not very strong in simultaneous adsorption,the Statistical thermodynamic(ST) model gives the best description of weaker adsorbed protein in simultaneous adsorption,the Steric mass-action(SMA) model is most appropriate multi-component model considering all different conditions for both proteins.

关 键 词：化工 进展 增刊 二二三 三七 二熟 布井 气石 

分 类 号：TQ01[化学工程]