Label-free detection of hybridization of oligonucleotides by oblique-incidence reflectivity difference method  

作　　者：YUAN Kun WANG Xu LU Heng WEN Juan LU HuiBin ZHOU YueLiang JIN Kui-Juan YANG GuoZhen LI Wei RUAN KangCheng 

机构地区：[1]Beijing National Laboratory for Condensed Matter Physics,Institute of Physics,Chinese Academy of Sciences,Beijing 100190,China [2]Key Laboratory of Proteomics,Institute of Biochemistry and Cell Biology,Shanghai Institutes for Biological Sciences,Chinese Academy of Sciences,Shanghai 200031,China

出　　处：《Science China(Physics,Mechanics & Astronomy)》2010年第8期1434-1437,共4页中国科学:物理学、力学、天文学(英文版)

基　　金：supported by the National Basic Research Program of China (Grant No. 2007CB935700)

摘　　要：The microarrays of 20-base oligonucleotide with different concentrations are detected before and after hybridization by the oblique-incidence reflectivity difference (OI-RD) method. The experimental results prove that OI-RD is a label-free method which can not only distinguish the concentration difference of oligonucleotides before and after the hybridization but also detect the hybridization of short oligonucleotides. At present the OI-RD method can detect 0.39 μmol/L 20-base oligonucleotide or less. These results suggest that the OI-RD method is a promising and potential technique for label-free detection of biological microarrays.The microarrays of 20-base oligonucleotide with different concentrations are detected before and after hybridization by the oblique-incidence reflectivity difference (OI-RD) method. The experimental results prove that OI-RD is a label-free method which can not only distinguish the concentration difference of oligonucleotides before and after the hybridization but also detect the hybridization of short oligonucleotides. At present the OI-RD method can detect 0.39 μmol/L 20-base oligonucleotide or less. These results suggest that the OI-RD method is a promising and potential technique for label-free detection of biological microarrays.

关 键 词：oblique-incidence reflectivity difference microarray detection label-free detection oligonucleotides microarray 

分 类 号：Q78[生物学—分子生物学]