Fish germ cells  

作　　者：XU HongYan1, LI MingYou1, GUI JianFang2 & HONG YunHan1,2 1Department of Biological Sciences, National University of Singapore, Singapore 119260, Singapore 2 State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China 

出　　处：《Science China(Life Sciences)》2010年第4期435-446,共12页中国科学（生命科学英文版）

基　　金：supported by the Biomedical Research Council of Singapore (Grant Nos. R-05-1-21-19-404, R-08-1-21-19-585 and SBIC-SSCC C-002-2007);the Ministry of Education of Singapore (Grant No. R-154-000-285-112);the National University of Singapore (Grant No. R-154-000-153-720);the National Key Basic Research Program of China (Grant Nos. 2004CB117406 and 2010CB126301)

摘　　要：Fish, like many other animals, have two major cell lineages, namely the germline and soma. The germ-soma separation is one of the earliest events of embryonic development. Germ cells can be specifically labeled and isolated for culture and transplan-tation, providing tools for reproduction of endangered species in close relatives, such as surrogate production of trout in salmon. Haploid cell cultures, such as medaka haploid embryonic stem cells have recently been obtained, which are capable of mimicking sperm to produce fertile offspring, upon nuclear being directly transferred into normal eggs. Such fish originated from a mosaic oocyte that had a haploid meiotic nucleus and a transplanted haploid mitotic cell culture nucleus. The first semi-cloned fish is Holly. Here we review the current status and future directions of understanding and manipulating fish germ cells in basic research and reproductive technology.Fish, like many other animals, have two major cell lineages, namely the germline and soma. The germ-soma separation is one of the earliest events of embryonic development. Germ cells can be specifically labeled and isolated for culture and transplan-tation, providing tools for reproduction of endangered species in close relatives, such as surrogate production of trout in salmon. Haploid cell cultures, such as medaka haploid embryonic stem cells have recently been obtained, which are capable of mimicking sperm to produce fertile offspring, upon nuclear being directly transferred into normal eggs. Such fish originated from a mosaic oocyte that had a haploid meiotic nucleus and a transplanted haploid mitotic cell culture nucleus. The first semi-cloned fish is Holly. Here we review the current status and future directions of understanding and manipulating fish germ cells in basic research and reproductive technology.

关 键 词：FISH GERM cell GERM plasm REPRODUCTION REPRODUCTIVE technology semi-cloning 

分 类 号：Q952[生物学—动物学]