DNA barcoding, phylogenetic relationships and speciation of snappers (genus Lutjanus)  

作　　者：WANG ZhongDuo1,2,3, GUO YuSong1,3, TAN Wei1, LI Lu1, TANG EnPu1, LIU ChuWu1,2,3 & LIU Yun2 1Fisheries College, Guangdong Ocean University, Zhanjiang 524025, China 2College of Life Science, Hunan Normal University, Changsha 410081, China 3Key Laboratory of Aquaculture in South China Sea for Aquatic Economic Animal of Guangdong Higher Education Institutes, Zhanjiang 524025, China 

出　　处：《Science China(Life Sciences)》2010年第8期1025-1030,共6页中国科学（生命科学英文版）

基　　金：supported by the National Science and Technology Pillar Program in the 11th Five-Year Plan Period of China (Grant No. 2007BAD29B03);the National Natural Science Foundation of China (Grant No. 30972253)

摘　　要：The phylogenetic relationships of 13 snapper species from the South China Sea have been established using the combined DNA sequences of three full-length mitochondrial genes (COI, COII and CYTB) and two partial nuclear genes (RAG1, RAG2). The 13 species (genus Lutjanus) were selected after DNA barcoding 72 individuals, representing 20 species. Our study suggests that although DNA barcoding aims to develop species identification systems, it may also be useful in the construction of phylogenies by aiding the selection of taxa. Combined mitochondrial and nuclear gene data has an advantage over an individual dataset because of its higher resolving power.The phylogenetic relationships of 13 snapper species from the South China Sea have been established using the combined DNA sequences of three full-length mitochondrial genes (COI, COII and CYTB) and two partial nuclear genes (RAG1, RAG2). The 13 species (genus Lutjanus) were selected after DNA barcoding 72 individuals, representing 20 species. Our study suggests that although DNA barcoding aims to develop species identification systems, it may also be useful in the construction of phylogenies by aiding the selection of taxa. Combined mitochondrial and nuclear gene data has an advantage over an individual dataset because of its higher resolving power.

关 键 词：PHYLOGENETIC relationship LUTJANUS SPECIATION DNA BARCODING 

分 类 号：Q951[生物学—动物学]