Cell-death-mode switch from necrosis to apoptosis in hydrogen peroxide treated macrophages  

作　　者：LIN XuZhu SUN TingZhe CAI MeiHong SHEN PingPing 

机构地区：[1]State Key Laboratory of Pharmaceutical Biotechnology,Department of Biochemistry,School of Life Science,Nanjing University,Nanjing 210093,China

出　　处：《Science China(Life Sciences)》2010年第10期1196-1203,共8页中国科学(生命科学英文版)

基　　金：supported by the National Natural Science Foundation of China (Grant No. 30870588);the Science Fund for Creative Research Groups (Grant No. 30821006);the Program for New Century Excellent Talents in University (Grant No. NCET-06-0445)

摘　　要：Cell death is typically defined either as apoptosis or necrosis. Because the consequences of apoptosis and necrosis are quite different for an entire organism, the investigation of the cell-death-mode switch has considerable clinical significance. The existence of a necrosis-to-apoptosis switch induced by hydrogen peroxide in macrophage cell line RAW 264.7 cells was confirmed by using flow cytometry and fluorescence microscopy. With the help of computational simulations, this study predicted that negative feedbacks between NF-κB and MAPKs are implicated in converting necrosis into apoptosis in macrophages exposed to hydrogen peroxide, which has significant implications.Cell death is typically defined either as apoptosis or necrosis. Because the consequences of apoptosis and necrosis are quite different for an entire organism, the investigation of the cell-death-mode switch has considerable clinical significance. The existence of a necrosis-to-apoptosis switch induced by hydrogen peroxide in macrophage cell line RAW 264.7 cells was confirmed by using flow cytometry and fluorescence microscopy. With the help of computational simulations, this study predicted that negative feedbacks between NF-κB and MAPKs are implicated in converting necrosis into apoptosis in macrophages exposed to hydrogen peroxide, which has significant implications.

关 键 词：macrophage hydrogen peroxide APOPTOSIS NECROSIS cell-death-mode switch 

分 类 号：Q255[生物学—细胞生物学]