Overexpression of the Rap2.4f transcriptional factor in Arabidopsis promotes leaf senescence  

作　　者：XU Heng WANG XueChen CHEN Jia 

机构地区：[1]State Key Laboratory of Plant Physiology and Biochemistry,College of Biological Sciences,China Agricultural University,Beijing 100193,China

出　　处：《Science China(Life Sciences)》2010年第10期1221-1226,共6页中国科学(生命科学英文版)

基　　金：supported by the National Basic Research Program of China (Grant No. 2006CB100100);the National Program of Trans-genic Plant Research of China (Grant No. 2009ZX08009-025B)

摘　　要：Senescence is a complex and highly regulated process. Leaf senescence is influenced by endogenous developmental and external environmental signals. In this work, we found that expression of an Ap2/DREB-type transcription factor gene, Arabidopsis Rap2.4f (At4g28140), was upregulated by salt, mannitol, and dark treatments. Constitutively overexpressing Rap2.4f under the control of the CaMV 35S promoter led to an increased chlorophyll degradation rate and upregulation of many senescence-associated genes in the transgenic Arabidopsis lines. Our results show that Rap2.4f is a positive regulator of senescence, promoting both developmental and dark-induced leaf senescence.Senescence is a complex and highly regulated process. Leaf senescence is influenced by endogenous developmental and external environmental signals. In this work, we found that expression of an Ap2/DREB-type transcription factor gene, Arabidopsis Rap2.4f (At4g28140), was upregulated by salt, mannitol, and dark treatments. Constitutively overexpressing Rap2.4f under the control of the CaMV 35S promoter led to an increased chlorophyll degradation rate and upregulation of many senescence-associated genes in the transgenic Arabidopsis lines. Our results show that Rap2.4f is a positive regulator of senescence, promoting both developmental and dark-induced leaf senescence.

关 键 词：Arabidopsis thaliana leaf senescence AP2 Rap2.4f 

分 类 号：Q943[生物学—植物学]