Kinetic characteristics of continuous flow polymerase chain reaction chip: A numerical investigation  

作　　者：WANG ShiYing1 & WANG Wei2,3 1 School of Physics, Peking University, Beijing 100871, China 2 Institute of Microelectronics, Peking University, Beijing 100871, China 3 National Key Laboratory of Science and Technology on Micro/Nano Fabrication, Beijing 100871, China 

出　　处：《Science China(Technological Sciences)》2010年第7期1967-1972,共6页中国科学（技术科学英文版）

基　　金：supported by the National Natural Science Foundation of China (Grant No. 60606014);Major State Basic Research Development Program (973 Program) (Grant No. 2009CB320300);the President Foundation of Peking University

摘　　要：Continuous flow PCR (polymerase chain reaction) chip holds impressive advantages compared to micro chamber PCR chip. In order to have better understanding of kinetic characteristics of continuous flow PCR chip, a comprehensive mathematical model is presented in this paper, including melting, annealing and extension phases of a typical PCR process which has the essence of a convection-diffusion-reaction system. Using this model, we can simulate the PCR process in series of reaction cycles. Numerical results show that the average sample velocity plays a significant role in affecting the amplification efficiency. Also, appropriate combination of the PCR mixture is important for high-quality DNA amplification. Giving a large initial DNA concentration range, the continuous flow PCR scheme holds excellent real-time detection ability theoretically. The present numerical model bridges the temperature distribution to the real DNA amplification, and thereby is able to successfully predict continuous flow PCR properties which are important for the chip design.Continuous flow PCR (polymerase chain reaction) chip holds impressive advantages compared to micro chamber PCR chip. In order to have better understanding of kinetic characteristics of continuous flow PCR chip, a comprehensive mathematical model is presented in this paper, including melting, annealing and extension phases of a typical PCR process which has the essence of a convection-diffusion-reaction system. Using this model, we can simulate the PCR process in series of reaction cycles. Numerical results show that the average sample velocity plays a significant role in affecting the amplification efficiency. Also, appropriate combination of the PCR mixture is important for high-quality DNA amplification. Giving a large initial DNA concentration range, the continuous flow PCR scheme holds excellent real-time detection ability theoretically. The present numerical model bridges the temperature distribution to the real DNA amplification, and thereby is able to successfully predict continuous flow PCR properties which are important for the chip design.

关 键 词：CONTINUOUS flow PCR CHIP NUMERICAL simulation convection-diffusion-reaction system 

分 类 号：O631.3[理学—高分子化学]