Isolation and differentiation of neural stem/progenitor cells from fetal rat dorsal root ganglia  

作　　者：GU Yun HU Nan LIU Jie DING Fei GU XiaoSong 

机构地区：[1]Medical School of Soochow University,Suzhou 215123,China [2]Jiangsu Key Laboratory of Neuroregeneration,Nantong University,Nantong 226001,China

出　　处：《Science China(Life Sciences)》2010年第9期1057-1064,共8页中国科学(生命科学英文版)

基　　金：supported by the National High Technology Research and Development Program of China (Grant No. 2006AA02A128);the National Natural Science Foundation of China (Grant No. 30670667);the Natural Science Foundation of Jiangsu Province (Grant No. BK2008010)

摘　　要：To find a promising alternative to neurons or schwann cells (SCs) for peripheral nerve repair applications,this study sought to isolate stem cells from fetal rat dorsal root ganglion (DRG) explants.Molecular expression analysis confirmed neural stem cell characteristics of DRG-derived neurospheres in terms of expressing neural stem cell-specific genes and a set of well-defined genes related to stem cell niches and glial fate decision.Under the influence of neurotrophic factors,bFGF and NGF,the neurospheres gave rise to neurofilament-expressing neurons and S100-expressing Schwann cell-like cells by different pathways.This study suggests that a subpopulation of stem cells that reside in DRGs is the progenitor of neurons and glia,which could directly induce the differentiation toward neurons,or SCs.To find a promising alternative to neurons or schwann cells (SCs) for peripheral nerve repair applications,this study sought to isolate stem cells from fetal rat dorsal root ganglion (DRG) explants.Molecular expression analysis confirmed neural stem cell characteristics of DRG-derived neurospheres in terms of expressing neural stem cell-specific genes and a set of well-defined genes related to stem cell niches and glial fate decision.Under the influence of neurotrophic factors,bFGF and NGF,the neurospheres gave rise to neurofilament-expressing neurons and S100-expressing Schwann cell-like cells by different pathways.This study suggests that a subpopulation of stem cells that reside in DRGs is the progenitor of neurons and glia,which could directly induce the differentiation toward neurons,or SCs.

关 键 词：dorsal root ganglia cell culture stem/progenitor cells IMMUNOCYTOCHEMISTRY induced differentiation 

分 类 号：Q42[生物学—神经生物学]