AtMYB103 is a crucial regulator of several pathways affecting Arabidopsis anther development  

作　　者：ZHU Jun ZHANG GuoQiang CHANG YuHua LI XiaoChuan YANG Jun HUANG XueYong YU QingBo CHEN Hui WU TianLong YANG ZhongNan 

机构地区：[1]College of Agriculture and Biotechnology,Shanghai Jiao Tong University,Shanghai 200240,China [2]College of Life and Environmental Sciences,Shanghai Normal University,Shanghai 200234,China [3]Shanghai Institute of Plant Physiology and Ecology,Chinese Academy of Sciences,Shanghai 200032,China

出　　处：《Science China(Life Sciences)》2010年第9期1112-1122,共11页中国科学(生命科学英文版)

基　　金：supported by the National Natural Science Foundation of China (Grant No. 30925007);the National Basic Research Program of China (Grant No. 2007CB947600)

摘　　要：Previous reports indicated that AtMYB103 has an important role in tapetum development,callose dissolution,and exine formation in A.thaliana anthers.Here,we further characterized its function in anther development by expression pattern analysis,transmission electron microscopy observation of the knockout mutant,and microarray analysis of downstream genes.A total of 818 genes differentially expressed between ms188 and the wild-type were identified by global expression profiling analysis.Functional classification showed that loss-of-function of AtMYB103 impairs cell wall modification,lipid metabolic pathways,and signal transduction throughout anther development.RNA in situ hybridization confirmed that transcription factors acting downstream of AtMYB103 (At1g06280 and At1g02040) were expressed in the tapetum and microspores at later stages,suggesting that they might have important roles in microsporogenesis.These results indicated that AtMYB103 is a crucial regulator of Arabidopsis anther development.Previous reports indicated that AtMYB103 has an important role in tapetum development,callose dissolution,and exine formation in A.thaliana anthers.Here,we further characterized its function in anther development by expression pattern analysis,transmission electron microscopy observation of the knockout mutant,and microarray analysis of downstream genes.A total of 818 genes differentially expressed between ms188 and the wild-type were identified by global expression profiling analysis.Functional classification showed that loss-of-function of AtMYB103 impairs cell wall modification,lipid metabolic pathways,and signal transduction throughout anther development.RNA in situ hybridization confirmed that transcription factors acting downstream of AtMYB103 (At1g06280 and At1g02040) were expressed in the tapetum and microspores at later stages,suggesting that they might have important roles in microsporogenesis.These results indicated that AtMYB103 is a crucial regulator of Arabidopsis anther development.

关 键 词：ARABIDOPSIS AtMYB103 tapetum development transcription factor MICROARRAY 

分 类 号：Q944.4[生物学—植物学]