Recent advances in proteolysis and peptide/protein separation by chromatographic strategies  

作　　者：ZHANG XiangMin1, LIU BaoHong1, ZHANG LiHua2, ZOU HanFa2, CAO Jing1, GAO MingXia1, TANG Jia1, LIU Yun1, YANG PengYuan1 & ZHANG YuKui2 1Department of Chemistry and Institutes of Biomedical Sciences, Fudan University, Shanghai 200433, China 2Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China 

出　　处：《Science China Chemistry》2010年第4期685-694,共10页中国科学（化学英文版）

基　　金：support from the Major State Basie Research Development Program (Grant No. 2007CB914100);the National Natural Science Foundation of China (Grant Nos. 20875016 & 20735005);Shanghai Projects (Grant Nos. 08DZ2293601 & B109)

摘　　要：This review gives a broad glance on the progress of recent advances on proteolysis and peptide/protein separation by chroma-tographic strategies in the past ten years, covering the main research in these areas especially in China. The reviewed research focused on enzymatic micro-reactors and peptide separation in bottom-up approaches, and protein and peptide separation in top-down approaches. The new enzymatic micro-reactor is able to accelerate proteolytic reaction rate from conventionally a couple of hours to a few seconds, and the multiple dimensional chromatographic-separation with various models or arrays could sufficiently separate the proteomic mixture. These advances have significantly promoted the research of protein/peptide separation and identification in proteomics.This review gives a broad glance on the progress of recent advances on proteolysis and peptide/protein separation by chroma-tographic strategies in the past ten years, covering the main research in these areas especially in China. The reviewed research focused on enzymatic micro-reactors and peptide separation in bottom-up approaches, and protein and peptide separation in top-down approaches. The new enzymatic micro-reactor is able to accelerate proteolytic reaction rate from conventionally a couple of hours to a few seconds, and the multiple dimensional chromatographic-separation with various models or arrays could sufficiently separate the proteomic mixture. These advances have significantly promoted the research of protein/peptide separation and identification in proteomics.

关 键 词：PROTEOLYSIS ENZYMATIC DIGESTION CHROMATOGRAPHIC separation PEPTIDE enrichment mass SPECTROMETRY 

分 类 号：Q51[生物学—生物化学]