A whisker-like carbon composite for the immobilization of laccase and its bioelectrochemistry  

作　　者：MA GuoXian1,2,ZHONG QingDong2 & LU TianHong3 1Department of Chemical Engineering,Shanghai Institute of Technology,Shanghai 200233,China 2School of Materials Science and Engineering,Shanghai University,Shanghai 200072,China 3Department of Chemistry,Nanjing Normal University,Nanjing 210097,China 

出　　处：《Science China Chemistry》2010年第6期1332-1336,共5页中国科学（化学英文版）

摘　　要：A novel mesoporous carbon/whisker-like carbon (MCWC) composite was used for the immobilization of laccase (Lac) and its bioelectrochemical behaviors were studied.It was confirmed by XPS that Lac was strongly adsorbed on the surface of the MCWC composite.The cyclic voltammetric results showed that the immobilized Lac underwent a direct quasi-reversible electrochemical reaction.The value of the electron transfer rate constant ks was estimated to be 0.770 s-1,indicating a reasonably fast electron transfer between the immobilized Lac and the underlying electrode.The surface concentration (I-) of Lac was estimated to be 2.730 × 10-12 mol/cm2.Further experimental results showed that the immobilized Lac displayed an appreciable electrocatalytic activity to the electrochemical reduction of O2.These properties could be attributed to the particular structure of loosely packed nanometer-scale carbon whiskers and the existence of a large amount of oxygen-containing groups.The immo- bilization method and the novel carrier (MCWC) may find new applications in fabricating the biocatalysts for biofuel cells.A novel mesoporous carbon/whisker-like carbon (MCWC) composite was used for the immobilization of laccase (Lac) and its bioelectrochemical behaviors were studied.It was confirmed by XPS that Lac was strongly adsorbed on the surface of the MCWC composite.The cyclic voltammetric results showed that the immobilized Lac underwent a direct quasi-reversible electrochemical reaction.The value of the electron transfer rate constant ks was estimated to be 0.770 s-1,indicating a reasonably fast electron transfer between the immobilized Lac and the underlying electrode.The surface concentration (I-) of Lac was estimated to be 2.730 × 10-12 mol/cm2.Further experimental results showed that the immobilized Lac displayed an appreciable electrocatalytic activity to the electrochemical reduction of O2.These properties could be attributed to the particular structure of loosely packed nanometer-scale carbon whiskers and the existence of a large amount of oxygen-containing groups.The immo- bilization method and the novel carrier (MCWC) may find new applications in fabricating the biocatalysts for biofuel cells.

关 键 词：LACCASE whisker-like carbon composite direct electrochemical reaction oxygen BIOELECTROCHEMISTRY 

分 类 号：Q814[生物学—生物工程]