FQ-PCR技术在儿童支原体肺炎早期诊断中的应用  

Early diagnosis of mycoplasma pneumonia infection in children by PCR technique

作　　者：黄建宏[1] 林心[1] 李文锋[1] 冯小三[1] 

机构地区：[1]广东省珠海市人民医院检验科,广东珠海519000

出　　处：《分子诊断与治疗杂志》2009年第1期18-19,共2页Journal of Molecular Diagnostics and Therapy

摘　　要：目的研究荧光定量PCR检测在儿童支原体肺炎(MP)感染早期诊断中的作用。方法利用荧光定量PCR检测呼吸系统受感染儿童咽拭子中肺炎支原体(MP)的DNA含量,并与血清中MP特异性抗体(MP-IgM)检测阳性结果比较。结果共检测540例,在3岁以下患儿组中,咽拭子中MP-DNA阳性率显著高于血清MP-IgM的阳性率(P<0.05),而3岁以上各组中两种方法阳性率无显著差异(P>0.05)。结论荧光定量PCR法可作为早期快速诊断儿童(尤其3岁以下患儿)MP呼吸系统感染的方法。Objective To explore the early diagnosis of mycoplasma pneumonia(MP) infection in Children. Methods Fluorescence quantitative polymerase chain reaction(FQ-PCR)technique were adopted respectively to detect the DNA of MP and mycoplasma pneumonia IgM antibody in serum samples in 1～10 infants with the prescribed diagnosis of respiratory tract infection. Results The positive rate in the results between MP-DNA and IgM antibody was statistically significant, In one to three years old children group(P<0.05). Conclusion MP is one of important pathogens that cause respiratory tract infection of children.FQ-PCR method is a rapid,sensitive and specific assessment in detecting the MP-DNA.

关 键 词：肺炎支原体 荧光定量PCR 呼吸道感染 

分 类 号：R725.6[医药卫生—儿科]