HBV前S1抗原与HBV-DNA、HBV-M的关系  

The relationship of HBV PreS1-Ag and HBV-DNA、HBV-M in patients with hepatitis B virus infection

作　　者：欧阳文婷[1] 曾智杰[1] 孙艳虹[1] 

机构地区：[1]中山大学附属第一医院检验医学部,广东广州510080

出　　处：《分子诊断与治疗杂志》2009年第1期20-22,共3页Journal of Molecular Diagnostics and Therapy

摘　　要：目的探讨乙型肝炎病毒前S1抗原(HBV PreS1-Ag)与HBV-DNA和HBV表面标志物(HBV-M)以及转氨酶之间的关系。方法从临床检测HBV-M的标本中筛出HBV阳性病例150例,采用ELISA法检测乙肝前S1抗原和荧光定量PCR法检测标本HBV-DNA,并与25例HBsAb(+)和5例HBV-M全阴性血清进行比较,同时采用酶法检测所有血清标本的天冬氨酸氨基转移酶(AST)和丙氨酸氨基转移酶(ALT)。结果HBV-DNA和HBV PreS1-Ag在HbeAg(+)组中检出率分别为100%和90.1%,在HbsAg(+)+HbeAb(+)+HbcAb(+)组中检出率为48.1%和40%。在HbsAg(+)+HbcAb(+)和单纯HbsAg(+)两组中,HBV-DNA与前S1抗原仍有一定检出率。HBV PreS1-Ag(+)组中,ALT与AST异常率分别占54.3%和46.7%。结论HBV-DNA检测为反映乙肝病毒复制最灵敏、特异的方法,乙肝前S1抗原检测成本低廉,与HBV-DNA的符合度较高,是对乙肝"两对半"和HBV-DNA测定的重要补充和加强,适合在没有条件开展HBV-DNA定量的广大基层医院推广。Objective To study the relationship between HBV PreS1-Ag、HBV-DNA、HBV-M and hepatic enzyme in patient infected with HBV. Methods The serum pre-S1 antigen and HBV-DNA were tested by ELISA and real-time fluorescent quantitative PCR(RT-PCR) in 150 patients infected with HBV screening HBV-M by ELISA method. At the mean time, 25 HBV-Ab(+) and 5 HBV-M negative samples were tested. Aspartate aminotransferase(AST) and alanine aminotransferase(ALT) were tested by rate method in all samples. Results The positive rates of the pre-S1 antigen and HBV-DNA were highest in HbeAg’s positive patients with different serology models of hepatitis B virus infection, which were 90.1% and 100% separately. The positive detection rates for pre-S1 antigen and HBV-DNA in the HbsAg(+)+HbeAb(+)+HbcAb(+) model were 40.2% and 48.1% separately. There still had a little positive rate of HBV-DNA and Pre S1-Ag in the HbsAg (+)+HbcAg(+) model and HbsAg(+) model. The abnormal rate of ALT and AST in the pre-S1 antigen’s positive model were higher than that in pre-S1 antigen’s negative model (ALT 54.3% and 0, AST 46.7% and 0). Conclusion The detection of HBV-DNA is the most sensitive and specific method reflecting the replication of hepatitis B virus in clinical. Hepatitis B virus Pre S1-Ag detection can be used as a supplement to hepatitis B markers and HBV-DNA detection. Especially hepatitis B virus Pre S1-Ag detection is suitable to widely been applied in grass-roots hospital lack of condition for real-time fluorescent quantitative PCR.

关 键 词：HBV-M HBV-DNA 前S1抗原 

分 类 号：R446.6[医药卫生—诊断学] R450[医药卫生—临床医学]