通心络治疗与SAMP8早期脑内淀粉样病变改善的相关性研究  

Relationships Between Tongxinluo Administration and Early Stage of Amyloid Pathogenesis in SAMP8 Mice Brain

作　　者：李亦清[1] 张莹[2] 何金生[2] 王宏涛 朱慧明 宋莉[1] 张志慧 周龙岗 魏刚 何奇龙 王鑫[2] 

机构地区：[1]山西大学生物技术研究所,山西太原030006 [2]北京交通大学理学院生命科学与生物工程研究院,北京100044 [3]河北以岭医药研究院,河北石家庄050035

出　　处：《现代生物医学进展》2009年第24期4609-4612,4592,共5页Progress in Modern Biomedicine

基　　金：国家重点基础研究发展计划(973计划)(2005CB523301)

摘　　要：目的:研究中药通心络(TXL)与治疗SAMP8小鼠早期脑内淀粉样病理变化改善的相关性。方法:取4月龄雄性SAMP8小鼠,随机分为6组:SAMP8(空白对照)组、石杉碱甲(阳性对照)组、TXL低剂(治疗)组、TXL中剂(治疗)组、TXL高剂(治疗)组、SAMR1(对照)组,每组15只。于第八周末,检测海马神经元染色质聚缩情况、皮质和海马糖原沉积情况、脑内淀粉样物质沉积情况、小鼠皮质Aβ1-42寡聚体的水平。结果:(1)HE染色结果显示SAMP8组有明显的核深染现象,通心络治疗组有改善;(2)与SAMP8对照组相比,TXL中剂组、TXL高剂组淀粉样沉积均有显著性差异(P<0.01);(3)与SAMP8组相比,TXL低剂组(P<0.05)、TXL中剂组(P<0.01)和TXL高剂组(P<0.05)PAS阳性颗粒(acid-Schiff(PAS)-positive granular structures,PGS)显著减少;(4)与SAMP8相比,TXL各治疗组Aβ42寡聚体水平均有显著性降(P<0.01);(5)TXL治疗剂量与淀粉样沉积、糖原沉积具有相关性,TXL中剂量治疗即可达到改善的效果,而Aβ42寡聚体聚集水平与TXL剂量有显著的线性负相关(r=-0.856,P<0.01)。结论:通心络治疗能够明显改善SAMP8小鼠早期脑内淀粉样病变。Objective: To investigate the effects of Tinxinluo treament on amyloid pathological changes in early stage of SAMP8 mice brain. Methods: Four month-old male SAMP8 mice were divided randomly into 6 groups (n=15): SAMP8 vehicle group, HuperzineA treatment group(positive control), low-dose of Tongxinluo group (TXL-L), 4 medium-dose of Tongxinluo group(TXL-M), 5 high-dose of Tongxinluo group (TXL-H), and SAMR1 group. At the end of eighth week, the chromatin condensation in Hippocampal neurons, the brain amyloid aggregates, the glycogen deposition in cortex and hippocampus, the monitoring Aβ1-42 oligermers level were detected. The soft ware SPSS15.0 were employmented as statistic tool. Results: (1) HE staining showed SAMP8 group had obviously nuclear condensation in Hippocampus, while TXL treatment groups had improvement; (2) Compared with the SAMP8 group, amyloid deposition of TXL-M group (P<0.01) and TXL-H group (P<0.01)were decreased significantly; (3) Compared with the SAMP8 group, PGS were reduced in all of the therapy groups including TXL-L group (P<0.05), TXL-M group (P<0.01) and TXL-H group (P<0.05); (4) Compared with the SAMP8 group, Aβ42 oligermer level declined Significantly (P<0.01) in All of Tongxinluo treatment groups; (5) TXL dose and Congo red signal, PAS staining are relevant, TXL-M dose is enough for this effect, while the level of Aβ42 oligomers content had a significant negative linear dose-dependent manner (r= -0.856, P<0.01). Conclusions: Tongxinluo treatment can improve the early stage amyloid padthogenesis in SAMP8 mice.

关 键 词：通心络超微粉 阿尔茨海默症 SAMP8小鼠 淀粉样沉积 

分 类 号：R285.5[医药卫生—中药学]