大鼠脾切除后自体脾细胞和脾片联合移植体液免疫学研究  

Humoral immunity research on combination transplantation of antogeneic Splenic cell and slices after rat’s splenectomy

作　　者：郭利光[1] 孙晓光[1] 欧阳军[1] 

机构地区：[1]石河子大学医学院第一附属医院急诊外科,新疆石河子832008

出　　处：《现代生物医学进展》2009年第24期4649-4651,共3页Progress in Modern Biomedicine

摘　　要：目的:通过建立脾细胞移植和脾片联合移植动物模型来观察脾片移植早期体液免疫功能恢复状况。方法:Wistar大鼠48只,随机分为4组,其中A组为对照组,行脾切除术,B组为脾片移植组,C组为脾细胞移植组,D组为脾细胞脾片联合移植组,在建立模型前、及模型成功后1周、4周、8周抽血,检测外周血IgM、IgG、IgA水平并进行比较。结果:A组脾切除后IgM逐渐降低(P<0.05);B组移植后8周IgM接近正常(P>0.05);C组移植后IgM到4周逐渐接近正常(P>0.05);而8周逐渐降低(P<0.05);D组术后4周和8周接近正常(P>0.05);在术后1、4周C和D组与A和B组有差异(P<0.05),在术后8周B和D组与A和C组有差异(P<0.05)。而IgG、IgA在四组术前术后变化不大(P>0.05)。结论:在脾片移植的基础上联合脾细胞移植能弥补脾片移植早期的部分体液免疫功能。Objective: To observe functional restoration of pristine humoral immunity aft-er splenic sliices autotransplantation by establishing animal model on combination trantran-splantation of antogeneic Splenic cell and slices. Methods:48 wistar rats are randomly divid ed into four groups,A group: splenectomy. B group: splenic slices transplantation. C group: spleen cell transplantation. D group: combination transplantation. Before animal moddel and within 1、4、8weeks after stablishing animal model, peripheral blood is drewn and detected the level of peripheral blood IgM,At the same time ,the results are compared. Result: In A group postoperative immunoglobulin M became gradually lower(P<0.05); In B group immunoglobulin M got close common at the postoperative 8th week (P>0.05);In C group immunoglobulin M got close common at the postoperative 4th week(P>0.05),but became gradually lower at the postoperative 8th week (P<0.05); In D group immunoglobulin M got close common at the postoperative 4th and 8th week (P>0.05); Statistically difference was detected between the C、D and A、B groups for immunoglobulin M at the postoperative 1th and 4th week (P<0.05), Statistically difference was detected between the B、D and A、C groups for immunoglobulin M at the postoperative 8th week (P<0.05). But No statistically difference was detected among all the groups for immunoglobulin G and A (P>0.05).Conclusion:Combination trantransplantation of antogeneic Splenic cell and slices can retrieve partial pristine humoral immune function.

关 键 词：脾切除 自体脾组织移植 脾脏 

分 类 号：R657.6[医药卫生—外科学]