HPLC-ELSD法测定消栓通颗粒中黄芪甲苷的含量  

Determination of Astragoloside Ⅳ in Xiaoshuantong Granules by HPLC-ELSD

作　　者：李滨[1] 齐凤琴[1] 刘石磊[1] 邹存珍[1] 

机构地区：[1]黑龙江中医药大学,黑龙江哈尔滨150040

出　　处：《现代生物医学进展》2009年第24期4765-4766,共2页Progress in Modern Biomedicine

摘　　要：目的:建立测定消栓通颗粒中黄芪甲苷含量的新方法。方法:采用高效液相色谱-蒸发光散射检测法测定,色谱柱为kromasilC18柱,以乙腈-水(32:68)为流动相,流速为1.0ml/min,ELSD条件为飘逸管温度为105℃,载气流速为2.5L/min;测定消栓通颗粒中黄芪甲苷的含量。结果:黄芪甲苷在1.08μg-6.48μg范围内与峰面积线性关系良好(r=0.9999),平均加样回收率为100.2%,RSD为0.93%。结论:方法灵敏、可靠、准确、重复性好,可作为消栓通颗粒的质量控制方法。Objective:To determine the content of Astragoloside IV in Xiaoshuantong Granules by HPLC-ELSD. Methods:HPLC- ELSD separation was achieved. Results:The astragaloside Ⅳ showed good linearity (r=0.9999)in the range of 1.08μg-6.48μg, the average recovery was 100.2% with RSD 0.93%. Conclusions:The method is sensitive,reliable,accurate and repeatability,it can be used for quality control method of Xiaoshuantong granules.

关 键 词：消栓通颗粒 黄芪甲苷 HPLC-ELSD 

分 类 号：R286[医药卫生—中药学]