病毒病抗性不同的大豆品种及其F_1代过氧化物酶酯酶同工酶分析  

ANALYSIS OF THE PEROXIDASE ISOZYME AND LIPASE ISOZYME ZYMOGRAMS IN SOYBEAN VARIETIES AND THEIR F_1 HYBRIDS WITH DIFFERENT DEGREE OF RESISTANCE TO SMV

作　　者：陈怡[1] 栾晓燕[1] 黄承运 谷秀芝[1] 杜维广[1] 张桂茹[1] 满为群[1] 刘丽君[1] 

机构地区：[1]黑龙江省农业科学院大豆研究所

出　　处：《大豆科学》1993年第1期30-36,共7页Soybean Science

基　　金：国家自然科学基金

摘　　要：本研究对大豆病毒病抗性不同的大豆杂交亲本及F_1代,接种SMV_1号株系后的过氧化物酶和酯酶同工酶的变化进行了分析。结果表明,抗病与感病亲本及其F_1代接种后的同工酶谱带存在着明显的差异。感病亲本及F_1代酶的活性增强,酶带数较多,而且部分酶带变宽,颜色变深。抗病亲本则不发生变化。各组合F_1代的酶谱与双亲有关,抗病×抗病的F_1代酶谱与双亲完全相同,抗病×感病的F_1代酶谱偏向抗性亲本,感病×感病的F_1代产生一个双亲所不具备的小分子新杂种酶带。未接种的感病亲本酶谱与抗病亲本相同。植株内部同工酶的变化与成株抗性相一致。因此,我们认为过氧化物酶同工酶,可作为鉴定大豆抗感病毒病的生化指标之一。The changes of the peroxidase isozyme and lipase isozyme zyrnograms were analy-sised on soybean varieties and their F1 hybrids with different resistance by inoculating SMV No. 1 strain. The result indicated that there were obvious difference on the parents and their F1 hybrids. The zymic activity of the inocalated susceptible parents and F1 hybrids was obviously higher and the zymic bands were more, some of the bands were wider and the color was more dark than those of the resistant The zymic bands of F1 hybrids of resistant x resistant parents were complete the same with their parents. The F1 hybrids of susceptible x susceptible parents produced 1 new hybrid zymic band which their parents were in shortage. The F1 hybrids of resistant x susceptible parent showed the zymic bands resenbling their resistant parent. The changes of zymograms within the plant were agree with the plant resistance. Therefore, we think peroxidase isozyme can be used as one biochemical indicater for evaluating resistance to SMV in soybean.

关 键 词：大豆 品种 抗病性 病毒病 同功酶 

分 类 号：S565.103.2[农业科学—作物学]