多效唑对异养小球藻USTB-01生长和蛋白含量的效应  

Effect of paclobutrazol on the growth and protein content of Chlorella sp USTB-01 in heterotrophic culture

作　　者：杨帅[1] 许倩倩[1] 王子敬[1] 杨晓静[1] 闫海[1] 

机构地区：[1]北京科技大学应用科学学院生物科学与技术系,北京100083

出　　处：《现代化工》2009年第S2期160-162,共3页Modern Chemical Industry

基　　金：中国石油科技创新基金(2009D-5006);北京科技大学冶金工程研究院研究基金联合资助项目(2009-05)

摘　　要：主要研究了生长调节剂多效唑对小球藻USTB-01生长和蛋白含量的效应,结果表明在其质量浓度0~50 mg/L内,随多效唑浓度的增加,抑制小球藻USTB-01生长的作用逐渐加强,但小球藻USTB-01蛋白质含量却逐渐从25%提高到37%,提高了48%;进一步采用先使小球藻快速生长,然后提高蛋白质含量的控制战略,在小球藻处于生长指数后期的第4天添加多效唑,不仅获得了较高浓度的藻生物量,而且藻蛋白质含量可以更进一步提高到47%,已经达到自养培养小球藻蛋白质含量的水平。The effect of paclobutrazol(PP333) on both the growth and the protein content of Chlorella USTB-01 is investigated,which show that the growth of Chlorella USTB-01 is inhibited,but the content of protein in Chlorella USTB-01 is increased to 48% from 25% to 37% with the increase of initial PP333 from 0 to 50 mg/L.The control strategy that firstly let the fast growth and then increased the protein content of Chlorella USTB-01 is adopted and PP333 is added into culture at the late exponential growth phase of day 4,both high cell density and high protein content of 47% are obtained,which reaches the protein level of Chlorella in autotrophic culture.

关 键 词：小球藻USTB-01 多效唑 生长 蛋白质 

分 类 号：Q943.1[生物学—植物学]